European Biophysics Journal

, Volume 42, Issue 11–12, pp 851–855 | Cite as

Dynamics of adhesion molecule domains on neutrophil membranes: surfing the dynamic cell topography

  • Thomas R. Gaborski
  • Michael N. Sealander
  • Richard E. Waugh
  • James L. McGrath
Biophysics Letter

Abstract

Lateral organization and mobility of adhesion molecules play a significant role in determining the avidity with which cells can bind to target cells or surfaces. Recently, we have shown that the lateral mobility of the principal adhesion molecules on neutrophils is lower for rolling associated adhesion molecules (RAAMs: L-selectin and PSGL-1) than for β2 integrins (LFA-1 and Mac-1). Here we report that all four adhesion molecules exhibit distinct punctate distributions that are mobile on the cell surface. Using uniform illumination image correlation microscopy, we measure the lateral mobility of these topologically distinct domains. For all four molecules, we find that diffusion coefficients calculated from domain mobility agree with measurements we made previously using fluorescence recovery after photobleaching. This agreement indicates that the transport of receptors on the surface of the resting neutrophil is dominated by the lateral movement of domains rather than individual molecules. The diffusion of pre-assembled integrin domains to zones of neutrophil/endothelial contact may provide a mechanism to facilitate high avidity adhesion during the earliest stages of firm arrest.

Keywords

Luekocytes Inflammation Adhesion cascade Fluorescence microscopy 

Supplementary material

249_2013_931_MOESM1_ESM.doc (285 kb)
Supplementary material 1 (DOC 285 kb)
249_2013_931_MOESM2_ESM.mov (964 kb)
Supplementary material 2 (MOV 964 kb)

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Copyright information

© European Biophysical Societies' Association 2013

Authors and Affiliations

  • Thomas R. Gaborski
    • 1
    • 2
  • Michael N. Sealander
    • 3
  • Richard E. Waugh
    • 1
  • James L. McGrath
    • 1
  1. 1.Department of Biomedical EngineeringUniversity of RochesterRochesterUSA
  2. 2.Department of Biomedical EngineeringRochester Institute of TechnologyRochesterUSA
  3. 3.Department of Electrical and Computer EngineeringUniversity of RochesterRochesterUSA

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