Abstract
We have performed a quantitative X-ray absorption fine structure analysis of bacteriorhodopsin in purple membrane patches and in lipidic cubic phases regenerated with Mn2+. Lipidic cubic phases and purple membrane results have been compared, demonstrating that the lipidic cubic phase process does not introduce relevant distortions in the local geometry of the cation binding sites. For both samples, we have observed similarities for Mn2+ coordination in terms of type, number, and average distances of surrounding atoms, indicating a first coordination shell composed by 6 O atoms, and 3/4 C atoms located in the second coordination shell.
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Acknowledgments
We acknowledge the European Synchrotron Radiation Facility for provision of synchrotron radiation facilities and we would like to thank Dr. Pieter Glatzel for assistance in using the ID26 beam line. This work was supported by a Dirección General de Investigación grant BFU2006-04656/BMC, BFU2009-08758, and a Marie Curie International Outgoing Fellowship to A.P.-M. within the 7th European Community Framework Programme (PIOF-GA-2009-237120).
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A. Perálvarez-Marín and F. Sepulcre contributed equally.
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Perálvarez-Marín, A., Sepulcre, F., Márquez, M. et al. Combination of extended X-ray absorption fine structure spectroscopy with lipidic cubic phases for the study of cation binding in bacteriorhodopsin. Eur Biophys J 40, 1007–1012 (2011). https://doi.org/10.1007/s00249-011-0714-3
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DOI: https://doi.org/10.1007/s00249-011-0714-3