Abstract
The interaction of the synthetic antimicrobial peptide P5 (KWKKLLKKPLLKKLLKKL-NH2) with model phospholipid membranes was studied using solid-state NMR and circular dichroism (CD) spectroscopy. P5 peptide had little secondary structure in buffer, but addition of large unilamellar vesicles (LUV) composed of dimyristoylphosphatidylcholine (DMPC) increased the β-sheet content to ~20%. Addition of negatively charged LUV, DMPC–dimyristoylphosphatidylglycerol (DMPG) 2:1, led to a substantial (~40%) increase of the α-helical conformation. The peptide structure did not change significantly above and below the phospholipid phase transition temperature. P5 peptide interacted differently with DMPC bilayers with deuterated acyl chains (d54-DMPC) and mixed d54-DMPC–DMPG bilayers, used to mimic eukaryotic and prokaryotic membranes, respectively. In DMPC vesicles, P5 peptide had no significant interaction apart from slightly perturbing the upper region of the lipid acyl chain with minimum effect at the terminal methyl groups. By contrast, in the DMPC–DMPG vesicles the peptide increased disorder throughout the entire acyl chain of DMPC in the mixed bilayer. P5 promoted disordering of the headgroup of neutral membranes, observed by 31P NMR. However, no perturbations in the T 1 relaxation nor the T 2- values were observed at 30°C, although a slight change in the dynamics of the headgroup at 20°C was noticeable compared with peptide-free vesicles. However, the P5 peptide caused similar perturbations of the headgroup of negatively charged vesicles at both temperatures. These data correlate with the non-haemolytic activity of the P5 peptide against red blood cells (neutral membranes) while inhibiting bacterial growth (negatively charged membranes).
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Acknowledgments
DIF is the recipient of an Australian Postgraduate Award. JDG is the recipient of an Australian Research Council Future Fellowship (FT0991558).
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D. I. Fernandez and M.-A. Sani contributed equally to the publication.
Membrane-active peptides: 455th WE-Heraeus-Seminar and AMP 2010 Workshop.
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Fernandez, D.I., Sani, MA., Gehman, J.D. et al. Interactions of a synthetic Leu–Lys-rich antimicrobial peptide with phospholipid bilayers. Eur Biophys J 40, 471–480 (2011). https://doi.org/10.1007/s00249-010-0660-5
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DOI: https://doi.org/10.1007/s00249-010-0660-5