Abstract
Due to diffraction, the resolution of imaging emitted light in a fluorescence microscope is limited to about 200 nm in the lateral direction. Resolution improvement by a factor of two can be achieved using structured illumination, where a fine grating is projected onto the sample, and the final image is reconstructed from a set of images taken at different grating positions. Here we demonstrate that with the help of a spatial light modulator, this technique can be used for imaging slowly moving structures in living cells.
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Acknowledgments
This work was funded by the Medical Research Council, Carl Zeiss MicroImaging GmbH and the International Agency for Atomic Energy.
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This article has been submitted as a contribution to the Festschrift entitled “Uncovering cellular sub-structures by light microscopy” in honour of Professor Cremer’s 65th birthday.
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Hirvonen, L.M., Wicker, K., Mandula, O. et al. Structured illumination microscopy of a living cell. Eur Biophys J 38, 807–812 (2009). https://doi.org/10.1007/s00249-009-0501-6
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DOI: https://doi.org/10.1007/s00249-009-0501-6