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Acceptor-photobleaching FRET analysis of core kinetochore and NAC proteins in living human cells

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Abstract

Faithful chromatin segregation is mediated and controlled by the kinetochore protein network which assembles at centromeres. In this study, the neighbourhood relations of inner kinetochore and nucleosome-associated complex (NAC) proteins were analysed in living human interphase cells by acceptor photobleaching FRET. The data indicate that CENP-U is in close vicinity to CENP-I as well as to CENP-B and that CENP-M is close to CENP-T.

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Acknowledgments

We thank N. Klöcker, D. Foltz, I. Cheeseman, M. Coppey-Moisan and N. Audugé for the kind gift of plasmids and the Deutsche Forschungs-Gemeinschaft (DFG) for support (SPP 1128).

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Authors and Affiliations

  1. Department of Molecular Biology, Leibniz-Institute for Age Research, Fritz Lipmann Institute, Beutenbergstr. 11, 07745, Jena, Germany

    D. Hellwig, C. Hoischen, T. Ulbricht & Stephan Diekmann

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  1. D. Hellwig
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  2. C. Hoischen
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  3. T. Ulbricht
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  4. Stephan Diekmann
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Correspondence to Stephan Diekmann.

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This article has been submitted as a contribution to the Festschrift entitled “Uncovering cellular sub-structures by light microscopy” in honour of Professor Cremer’s 65th birthday.

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Hellwig, D., Hoischen, C., Ulbricht, T. et al. Acceptor-photobleaching FRET analysis of core kinetochore and NAC proteins in living human cells. Eur Biophys J 38, 781–791 (2009). https://doi.org/10.1007/s00249-009-0498-x

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  • DOI: https://doi.org/10.1007/s00249-009-0498-x

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