Abstract
A novel injectable polysaccharide system based on calcium Alginate (Ca-Alg) hydrogel and two Dextran methacrylate derivatives (DexMA) was recently developed. The resulting Interpenetrating Polymer Network showed a synergistic mechanical behavior that can be exploited to target the hydrogel properties towards specific biomedical needs. In the present paper, hydrogels composed of 3% (w/v) Ca-Alg and Dextran (Mw 40 × 104 and 500 × 104), derivatized with methacrylic groups (derivatization degrees 5 and 30%) at concentrations 5% (w/v), were characterized. The data reported here evidenced that Mw and derivatization degree of Dex chains can deeply affect the mechanical as well a model protein (Horseradish peroxidase) delivery rate. The enzymatic activity of such model protein was never significantly altered by the adopted experimental conditions.
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Acknowledgments
Dr. Silvia Chichiarelli is acknowledged for her valuable contribution to the discussion of the enzymatic data. Financial support from FIRB, “Fondo per gli Investimenti della Ricerca di Base, Research Program: Ricerca e Sviluppo del Farmaco” (CHEM-PROFARMA-NET), grant no. RBPR05NWWC_003 is acknowledged.
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Proceedings of the XIX Congress of the Italian Society of Pure and Applied Biophysics (SIBPA), Rome, September 2008.
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Pescosolido, L., Miatto, S., Di Meo, C. et al. Injectable and in situ gelling hydrogels for modified protein release. Eur Biophys J 39, 903–909 (2010). https://doi.org/10.1007/s00249-009-0440-2
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DOI: https://doi.org/10.1007/s00249-009-0440-2