Abstract
The translational displacement of the cytoplasmic water in Elodea stem cells resulting from protein motor activity was measured using the NMR method. A 24-h treatment with vincristine results in a reduction of the translational displacement of the cytoplasmic water. With a constant cytoplasmic streaming velocity, the dynamics of the translational displacement of the cytoplasmic water under the effect of taxol are characterized by a continuous increase at a concentration of 0.05 mM, and reaching a plateau at a concentration of 0.5 mM.
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References
Anisimov AV, Vorob’ev VN, Zholkevich VN (2003) The contribution of the actomyosin motor to intracellular water transport in Elodea canadensis. Dokl Biochem Biophys 392:292–293
Anisimov AV, Ionenko IF, Romanov AV (2004) Spin-echo NMR study of the translational water diffusion selectively along the apoplast and the cytoplasmic and vacuolar symplasts of plants. Biophysics 49:816–821
Gupta S, Bhattacharyya B (2003) Antimicrotubular drugs binding to vinca domain of tubulin. Mol Cell Biochem 253:41–47
Hasezawa S, Sano T, Nagata T (1998) The role of microfilaments in the organization and orientation of microtubules during the cell cycle transition from M phase toG1 phase in tobacco BY-2 cells. Protoplasma 202:105–114
Kimura Y, Toyoshima N, Hirakawa N, Okamoto K, Ishijima A (2003) A kinetic mechanism for the fast movement of Chara myosin. J Mol Biol 328:939–950
Ibrahim NK, Desai N, Legha S, Soon-Shiong P, Theriault RL, Rivera E, Esmaeli B, Ring SE, Bedikian A, Hortobagyi GN, Ellerhorst JA (2002) Phase I and pharmacokinetic study of ABI-007, a cremophor-free, protein-stabilized, nanoparticle formulation of paclitaxel. Clin Cancer Res 8:1038–1044
Kobayashi H, Fukuda H, Shibaoka H (1988) Interrelation between the spatial disposition of actin filaments and microtubules during the differentiation of tracheary elements in cultured Zinnia cells. Protoplasma 143:29–37
Luby-Phelps K (2000) Cytoarchitecture and physical properties of cytoplasm: volume, viscosity, diffusion, intracellular surface area. Int Rev Cytol 192:189–221
Minin AA, Kulik AV (2004) Intracellular transport. The principles of regulation. Usp Biol Khim 44:225–262 (in Russian)
Morimatsu M, Nakamura A, Sumiyosh H, Sakabe N, Taniguchi H, Kohama K, Higashi-Fujime S (2000) The molecular structure of the fastest myosin from green algae, Chara. Biochem Biophys Res Commun 270:147–152
Pickard WF (2003) The role of cytoplasmic streaming in symplastic transport. Plant Cell Environ 26:1–15
Steiskal D, Tanner JE (1965) Self-diffusion measurements: spin-echoes in the presense of a time dependent field gradient. J Chem Phys 42:288–292
Schiff PB, Fant J, Horwitz SB (1979) Promotion of microtubule assembly in vitro by taxol. Nature 277:665–667
Schiff PB, Horwitz SB (1980) Taxol stabilizes microtubules in mouse fibroblast cells. Proc Natl Acad Sci USA 77:1561–1565
Takesue K, Shibaoka H (1998) The cyclic reorientation of cortical microtubules in epidermal cells of azuki bean epicotyls: the role of actin filaments in the progression of the cycle. Planta 205:539–546
Tanner JE (1970) Use of the stimulated echo in NMR diffusion studies. J Chem Phys 52:2523–2526
Tiwary SC, Wick SM, Williamson RE, Gunning BES (1984) Cytoskeleton and integration of cellular function in cells higher plants. J Cell Biol 99:63–69
Tominaga M, Morita K, Yokota E, Simmen T (1997) Microtubules regulate the organization of actin filaments at the cortical region in root hair cells Hydrocharis. Protoplasma 199:83–92
Vorob’yev VN, Anisimov AV (1995) Aspects of the effect of rare earth ions on the velocity of rotational motion of the chloroplasts. Biophysics 40:537–541
Vorob’ev VN, Anisimov AV, Dautova NR (2004) Contribution of the actomyosin motor to the temperature-dependent translational diffusion of water by cytoplasmic streaming in Elodea canadensis cells. Protoplasma 224:195–199
Yokota E, McDonald AR, Liu B, Simmen T, Palevitz BA (1995) Localization of a 170 kDa myosin heavy chain in plant cells. Protoplasma 185:178–187
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Vorob’ev, V.N., Anisimov, A.V. & Dautova, N.R. Water diffusion in cytoplasmic streaming in Elodea internodal cells under the effect of antimitotic agents. Eur Biophys J 37, 975–978 (2008). https://doi.org/10.1007/s00249-008-0316-x
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DOI: https://doi.org/10.1007/s00249-008-0316-x