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The redox midpoint potential of the primary quinone of reaction centers in chromatophores of Rhodobacter sphaeroides is pH independent

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Abstract

The redox midpoint potential (E m) of the primary quinone of bacterial reaction centers, QA, in native membranes (chromatophores) measured by redox potentiometry is reported to be pH dependent (−60 mV/pH) up to a highly distinctive pK a (9.8 in Rba. sphaeroides) for the reduced state. In contrast, the E m of QA in isolated RCs of Rba. sphaeroides, although more variable, has been found to be essentially pH-independent by both redox potentiometry and by delayed fluorescence, which determines the free energy (ΔG P*A) of the P+Q −A state relative to P*. Delayed fluorescence was used here to determine the free energy of P+Q −A in chromatophores. The emission intensity in chromatophores is two orders of magnitude greater than from isolated RCs largely due to the entropic effect of antenna pigments “drawing out” the excitation from the RC. The pH dependence of ΔG P*A was almost identical to that of isolated RCs, in stark contrast with potentiometric redox titrations of QA. We considered that QA might be reduced by disproportionation with QH2 through the QB site, so the titration actually reflects the quinone pool, giving the −60 mV/pH unit dependence expected for the Q/QH2 couple. However, the parameters necessary to achieve a strong pH-dependence are not in good agreement with expected properties of QA and QB. We also consider the possibility that the time scale of potentiometric titrations allows the reduced state (Q −A ) to relax to a different conformation that is accompanied by stoichiometric H+ binding. Finally, we discuss the choice of parameters necessary for determining the free energy level of P+Q −A from delayed fluorescence emission from chromatophores of Rba. sphaeroides.

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Fig. 1
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Abbreviations

E m :

Redox midpoint potential

Q:

Quinone

RC:

(Bacterial) Reaction center

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Acknowledgments

This work was supported by NSF (MCB 03-44449 to CAW) and NKTH-OTKA (K-67850 to PM).

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  1. Department of Biophysics, University of Szeged, Egyetem utca 2, 6723, Szeged, Hungary

    Péter Maróti

  2. Center for Biophysics and Computational Biology, University of Illinois, MC-147, 607 South Mathews Street, Urbana, IL, 61801, USA

    Colin A. Wraight

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  1. Péter Maróti
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Correspondence to Colin A. Wraight.

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Regional Biophysics Conference of the National Biophysical Societies of Austria, Croatia, Hungary, Italy, Serbia, and Slovenia.

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Maróti, P., Wraight, C.A. The redox midpoint potential of the primary quinone of reaction centers in chromatophores of Rhodobacter sphaeroides is pH independent. Eur Biophys J 37, 1207–1217 (2008). https://doi.org/10.1007/s00249-008-0301-4

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