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Role of fructose in the adaptation of plants to cold-induced oxidative stress

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Abstract

This work presents findings, which indicate important role of fructose, fructose 6-phosphate (F6P), and fructose 1,6-bisphosphate (FBP) in preservation of homeostasis in plants under low temperature. Cold combined with light is known to incite increased generation of superoxide in chloroplasts leading to photoinhibition, but also an increased level of soluble sugars. In the present study, oxidative stress in pea leaves provoked by cold/light regime was asserted by the observed decrease of the level of oxidized form of PSI pigment P700 (P700+). Alongside, the increased antioxidative status and the accumulation of fructose were observed. The antioxidative properties of fructose and its phosphorylated forms were evaluated to appraise their potential protective role in plants exposed to chilling stress. Fructose, and particularly F6P and FBP exhibited high capacities for scavenging superoxide and showed to be involved in antioxidative protection in pea leaves. These results combined with previously established links implicate that the increase in level of fructose sugars through various pathways intercalated into physiological mechanisms of homeostasis represents important non-enzymatic antioxidative defense in plants under cold-related stress.

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Abbreviations

F6P:

Fructose 6-phosphate

FBP:

Fructose 1,6-bisphosphate

ROS:

Reactive oxygen species

OPP:

Oxidative pentose-phosphate pathway

PSI:

Photosystem I

SOD:

Superoxide dismutase

ABTS:

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)

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Acknowledgments

We are grateful to Mihajlo B. Spasić for constructive discussion. This work was supported by the Grants from the Ministry of Science of Republic of Serbia, 143016 and 143043.

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Correspondence to I. Spasojević.

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Regional Biophysics conference of the National Biophysical Societies of Austria, Croatia, Hungary, Italy, Serbia, and Slovenia.

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Bogdanović, J., Mojović, M., Milosavić, N. et al. Role of fructose in the adaptation of plants to cold-induced oxidative stress. Eur Biophys J 37, 1241–1246 (2008). https://doi.org/10.1007/s00249-008-0260-9

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  • DOI: https://doi.org/10.1007/s00249-008-0260-9

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