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Effect of redox mediators on the flash-induced membrane potential generation in Mn-depleted photosystem II core particles

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Abstract

An electrometrical technique was used to investigate flash-induced electron transfer reactions between Mn-depleted spinach photosystem II core particles incorporated into liposomes and redox mediators. Besides the fast increase in the transmembrane electric potential difference associated with electron transfer between the redox active tyrosine (YZ) and the primary quinone acceptor QA, an additional electrogenic phase was observed in the presence of N,N,N′N′-tetramethyl-p-phenylenediamine and 2,6-dichlorophenol-indophenol. The latter phase is attributed to vectorial electron transfer from the redox dye(s) to the protein-embedded YZ. The data obtained suggest an electrically isolated location of the YZ from the external water phase.

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Abbreviations

PS II:

Photosystem II

RC:

Reaction center

D1 and D2:

Polypeptides consisting the anchoring site of the main cofactors for PS II

P680 :

Primary electron donor in PS II

QA (QB):

Primary (secondary) plastoquinone acceptor

YZ :

Tyrosine-161 on D1 of PS II

OEC:

Oxygen-evolving complex

Δψ:

Transmembrane electric potential difference

MES:

2-(N-morpholino)ethanesulfonic acid

TMPD:

N,N,N′N′-tetrametyl-p-phenylenediamine

DCIP:

2,6-Dichlorophenol-indophenol

MB:

Methylene blue

τ :

Characteristic time constant

E m :

Midpoint redox potential

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Acknowledgments

We are grateful to Dmitry Cherepanov, for valuable discussions; Sergey Chamorovsky, for critical reading of the manuscript; and Andrey Zaspa, for technical assistance. The work was supported by grants from the Russian Foundation for Basic Research (06-04-48672 and 07-04-01050) and from Russian Federal Agency for Science and Innovation (02.512.11.2085).

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Authors and Affiliations

  1. A.N. Belozersky Institute of Physical-Chemical Biology, Moscow State University, Vorob’evy gory, GSP-2, 119991, Moscow, Russia

    Oksana A. Gopta, Anna A. Tyunyatkina, Vasiliy N. Kurashov, Alexey Yu. Semenov & Mahir D. Mamedov

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  1. Oksana A. Gopta
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  2. Anna A. Tyunyatkina
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  3. Vasiliy N. Kurashov
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  4. Alexey Yu. Semenov
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  5. Mahir D. Mamedov
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Correspondence to Alexey Yu. Semenov.

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Gopta, O.A., Tyunyatkina, A.A., Kurashov, V.N. et al. Effect of redox mediators on the flash-induced membrane potential generation in Mn-depleted photosystem II core particles. Eur Biophys J 37, 1045–1050 (2008). https://doi.org/10.1007/s00249-007-0231-6

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  • DOI: https://doi.org/10.1007/s00249-007-0231-6

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