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Thermotropic phase behavior of DPPC liposome systems in the presence of the anti-cancer agent ‘Ellipticine’

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Abstract

This letter presents our first results on the structural changes in DPPC multilamellar vesicles dispersed in water in the presence of the anti-cancer agent Ellipticine. The thermotropic phase transitions of the lamellar packing inside lipid vesicles were characterized in situ by small angle X ray diffraction. The results lead to the determination of a critical concentration value for drug loading on the vesicle system around 4% molar fraction of Ellipticine, an indication of the localization of the drug in the alkyl chains and the influence of the drug on the decreasing rate of the bilayer period after the main phase transition.

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References

  • Bernardes LA et al (1992) A small-angle X-ray-scattering workstation for national laboratory for synchrotron light. Rev Sci Instrum 63:1065–1067

    Article  ADS  Google Scholar 

  • Bota A, Kriechbaum M (1998) Prehistory in the pretransition range of dipalmitoylphosphatidylcholine/water system. Colloids Surfaces A 141:441–448

    Article  Google Scholar 

  • Cavalcanti LP et al (2006) Lipid model membrane for drug interaction study. Eur Biophys J 35:431–438

    Article  Google Scholar 

  • Kellermann G et al (1997) The small-angle X-ray scattering beamline of the Brazilian synchrotron light laboratory. J Appl Cryst 30:880–883

    Article  Google Scholar 

  • Kohn K et al (1975) Intercalative binding of ellipticine to DNA. Cancer Res35:71–76

    Google Scholar 

  • Mathe G et al (1998)Viral and immunologic follow up of 4 to 9 years of AIDS treatments by quadruple combinations of virostatics including integrase inhibitors applied in short sequences differing by drug rotation. Biomed Pharmacother 52:391–396

    Article  Google Scholar 

  • Sainsbury M (1990) Ellipticines. In: Wilman D (ed) The chemistry of antitumour agents, Chapman and Hall, New York, pp 410–435

    Google Scholar 

  • Singh MP et al (1994) High-field NMR and restrained molecular modeling studies on a DNA heteroduplex containing a modified apurinic abasic site in the form of covalently linked 9-aminoellipticinet. Biochemistry 33:10271–10285

    Article  Google Scholar 

  • Stiborová M et al (2001) The anticancer agent ellipticine on activation by cytochrome P450 forms covalent DNA adducts. Biochem Pharmac 62:1675–1684

    Article  Google Scholar 

  • Tardieu A et al (1973) Structure and polymorphism of the hydrocarbon chains of lipids: a study of lecithin-water phases. J Mol Biol 75:711–733

    Article  Google Scholar 

  • Torchillin VP, Weissig V (2003) Liposomes: a practical approach. 2nd edn. Oxford University Press, New York

    Google Scholar 

  • Wack DC, Webb WW (1989) Synchrotron X-ray study of the modulated lamellar Pβ′ in the lecithin-water system. Phys Rev A 40:2712–2730

    Article  ADS  Google Scholar 

Download references

Acknowledgment

We acknowledge the use of beamtime at the SAXS beamline of the Brazilian Synchrotron Light Laboratory (LNLS), T. S. Plivelic and G. Kellermann, the beamline staff, for the technical support and the CNPq Agency for funding this research.

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Authors and Affiliations

  1. Institute of Physics, State University of Campinas (UNICAMP), CP 6165, Campinas SP, 13083-970, Brazil

    Leide P. Cavalcanti & Iris L. Torriani

  2. Synchrotron Light National Laboratory (LNLS), CP 6192, Campinas SP, 13084-971, Brazil

    Leide P. Cavalcanti & Iris L. Torriani

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  1. Leide P. Cavalcanti
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  2. Iris L. Torriani
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Correspondence to Leide P. Cavalcanti.

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Cavalcanti, L.P., Torriani, I.L. Thermotropic phase behavior of DPPC liposome systems in the presence of the anti-cancer agent ‘Ellipticine’. Eur Biophys J 36, 67–71 (2006). https://doi.org/10.1007/s00249-006-0077-3

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  • DOI: https://doi.org/10.1007/s00249-006-0077-3

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