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Conditioning action of the environment on the protein dynamics studied through elastic neutron scattering

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Abstract

The dynamics of lysozyme in the picosecond timescale has been studied when it is in dry and hydrated powder form and when it is embedded in glycerol, glycerol–water, glucose and glucose–water matrices. The investigation has been undertaken through elastic neutron scattering technique on the backscattering spectrometer IN13. The dynamics of dry powder and embedded-in-glucose lysozyme can be considered purely vibrational up to 100 K, where the onset of an anharmonic contribution takes place. This contribution can be attributed to the activation of methyl group reorientations and is described with an Arrhenius trend. An additional source of anharmonic dynamics appears at higher temperatures for lysozyme in hydrated powders and embedded in glycerol, glycerol–water and glucose–water matrices. This second process, also represented with an Arrhenius trend, corresponds to the so-called protein dynamical transition. Both the temperature where such a transition takes place and the magnitude of the protein mean square displacements depend on the environment. The dynamical response of the protein to temperature is put in relationship with its thermal stability.

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Acknowledgements

The authors are grateful to ILL (Institut Laue-Langevin, Grenoble, France) and to the Italian-French CRG IN13 for providing beam time at the IN13 spectrometer.

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Authors and Affiliations

  1. Dipartimento di Fisica dell’Università di Perugia, CNR-INFM CRS SOFT, Via A. Pascoli, 06123, Perugia, Italy

    A. Paciaroni, E. Cornicchi, M. Marconi & G. Onori

  2. c/o Dipartimento di Chimica dell’Università di Perugia, CEMIN (Centro di Eccellenza per i Materiali Innovativi e Nanostrutturati), Via Elce di Sotto 8, 06123, Perugia, Italy

    A. Paciaroni, E. Cornicchi, M. Marconi & G. Onori

  3. CNR-INFM CRS SOFT, 00185, Rome, Italy

    A. De Francesco

  4. c/o Institut Laue-Langevin, Rue Jules Horowitz 6, 38042, Grenoble, France

    A. De Francesco

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  1. A. Paciaroni
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  2. E. Cornicchi
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Correspondence to A. Paciaroni.

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Paciaroni, A., Cornicchi, E., De Francesco, A. et al. Conditioning action of the environment on the protein dynamics studied through elastic neutron scattering. Eur Biophys J 35, 591–599 (2006). https://doi.org/10.1007/s00249-006-0073-7

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  • DOI: https://doi.org/10.1007/s00249-006-0073-7

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