A novel polymerase chain reaction (PCR) method is presented that utilizes Coriolis and centrifugal effects, produced by rotation of the sample disc, in order to increase internal circulatory rates, and with them temperature homogenization and mixing speeds. A proof of concept has been presented by testing a rapid 45-cycle PCR DNA amplification protocol. During the repeated heating and cooling that constitutes a PCR process, the 100 μL samples were rotated at a speed equivalent to an effective acceleration of gravity of 7,000 g. A cycle time of 20.5 s gave a total process time of 15 min to complete the 45 cycles. A theoretical and numerical analysis of the resulting flow, which describes the increased mixing and temperature homogenization, is presented. The device gives excellent reaction speed efficiency, which is beneficial for rapid PCR.
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Columbus RL, Helfer JL, Porte JJ, Wellman JA (1990) Temperature cycling cuvette. United States Patent 4,902,624
Ebmeier RJ, Whitney SE, Sarkar A, Nelson M, Padhye NV, Gogos G, Viljohen H (2004) Ranque-Hilsch vortex tube thermocycler for fast DNA amplification and real-time optical detection. Rev Sci Instrum 75:5356–5359
Hermann H, Knippschild C, Berka A (2004) Ultrafast DNA-amplification with a rapid PCR-system. BTi, November 2004
Malmquist M (2004) Homogenizing of small-volume mixtures by centrifugation and heating. United States Patent 6,783,993
Northrup MA, Mariella RP Jr, Carrano AV, Balch JW (1996) Silicon-based sleeve devices for chemical reactions. United States Patent 5,589,136
Petersen KE, McMillan WA, Kovacs GTA, Young SJ (1999) Reaction vessel for heat-exchanging chemical processes. United States Patent 5: 958–349
Skote M, Mårtensson G, Johansson AV (2005) Flow in a rapidly rotating cone-shaped PCR-tube. Internal report. Dept. of Mech., Royal Inst. of Tech., Stockholm, Sweden
Wittwer CT, Fillmore GC, Hillyard DR (1989) Automated polymerase chain reaction in capillary tubes with hot air. Nucleic Acids Res 17:4353–4357
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Mårtensson, G., Skote, M., Malmqvist, M. et al. Rapid PCR amplification of DNA utilizing Coriolis effects. Eur Biophys J 35, 453–458 (2006). https://doi.org/10.1007/s00249-006-0052-z
- Polymerase Chain Reaction
- Coriolis Force
- Temperature Homogenization
- Azimuthal Direction
- Boundary Condition