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Chronic hypoperfusion alters the content and structure of proteins and lipids of rat brain homogenates: a Fourier transform infrared spectroscopy study

European Biophysics Journal volume 33, pages 549–554 (2004)Cite this article

Abstract

Arteriovenous malformations (AVMs), masses of abnormal blood vessels which grow in the brain, produce high flow shunts that steal blood from surrounding brain tissue, which is chronically hypoperfused. Hypoperfusion is a condition of inadequate tissue perfusion and oxygenation, resulting in abnormal tissue metabolism. Fourier transform infrared (FTIR) spectroscopy is used in this study to investigate the effect of hypoperfusion on homogenized rat brain samples at the molecular level. The results suggest that the lipid content increases, the protein content decreases, the lipid-to-protein ratio increases, and the state of order of the lipids increases in the hypoperfused brain samples. FTIR results also revealed that, owing to hypoperfusion, not only the protein synthesis but also the protein secondary structure profile is altered in favor of β-sheets and random coils. These findings clearly demonstrate that, FTIR spectroscopy can be used to extract valuable information at the molecular level so as to have a better understanding of the effect of hypoperfusion on rat brain.

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Acknowledgements

This work was supported by The Scientific and Technical Research Council of Turkey, SBAG-2320 research fund, and TÜBİTAK-BAYG (The Scientific and Technical Research Council of Turkey – Directorate of Human Resources Development).

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Affiliations

  1. Department of Biology, Middle East Technical University, 06531 , Ankara, Turkey

    Neslihan Toyran, Gizem Dönmez & Feride Severcan

  2. Department of Radiation Oncology, Faculty of Medicine, Hacettepe University, 06100 , Sıhhıye, Ankara, Turkey

    Faruk Zorlu

  3. Department of Neurosurgery, Faculty of Medicine, Hacettepe University, 06100 , Sıhhıye, Ankara, Turkey

    Kamil Öğe

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  1. Neslihan Toyran
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  2. Faruk Zorlu
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  4. Kamil Öğe
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  5. Feride Severcan
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Correspondence to Feride Severcan.

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Toyran, N., Zorlu, F., Dönmez, G. et al. Chronic hypoperfusion alters the content and structure of proteins and lipids of rat brain homogenates: a Fourier transform infrared spectroscopy study. Eur Biophys J 33, 549–554 (2004). https://doi.org/10.1007/s00249-004-0396-1

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  • DOI: https://doi.org/10.1007/s00249-004-0396-1

Keywords

  • Brain
  • Hypoperfusion
  • Macromolecules
  • Secondary structure
  • Fourier transform infrared spectroscopy