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The strength of integrin binding between neutrophils and endothelial cells

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Abstract

The firm adhesion of activated polymorphonuclear neutrophils to endothelial cells in blood vessels is achieved through binding of the integrin intercellular adhesion molecule. To contribute to the better understanding of this adhesion step, our investigation is aimed at the relationship between integrin expression and the strength of neutrophil binding to endothelial cells. Flow cytometry and 3D scanning microscopy are used to study integrin expression and distribution, respectively. It is found that CD11b/CD18 integrin expression is localized in clusters distributed irregularly over the neutrophil surface. After cell activation, the cluster distribution polarizes, increasing the local CD11b/CD18 density concurrently with nearly doubled integrin expression. The neutrophil adhesion efficiency is measured in a flow chamber coated successively by various substrates, including endothelial cells in an activated state. Analysis of the flow dependence of the number of attached cells reveals the prevailing number of neutrophils with stronger binding to the endothelium when both cells are in the activated state in comparison with non-activated cells.

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References

  • Alon R, Chen S, Puri KD, Finger EB, Springer TA (1997) The kinetics of L-selectin tethers and mechanics of selectin-mediated rolling. J Cell Biol 138:1169–1180

    Article  CAS  PubMed  Google Scholar 

  • Arnaout MA (1990) Structure and function of the leukocyte adhesion molecules CD11/CD18. Blood 75:1037–1050

    CAS  PubMed  Google Scholar 

  • Chen S, Springer TA (1999) An automatic braking system that stabilizes leukocyte rolling by an increase in selectin bond number with shear. J Cell Biol 144:185–200

    Article  CAS  PubMed  Google Scholar 

  • Clark AC, Brugge JS (1995) Integrins and signal transduction pathways: the road taken. Science 268:233–238

    CAS  PubMed  Google Scholar 

  • Diacovo TG, Roth SJ, Buccola JM, Bainton DF, Springer TA (1996) Neutrophil rolling, arrest and transmigration across activated, surface-adherent platelets via sequential action of P-selectin and the β2-integrin CD11b/CD18. Blood 88:146–157

    CAS  PubMed  Google Scholar 

  • Diamond MS, Springer TA (1994) The dynamic regulation of integrin adhesiveness. Curr Biol 4:506–517

    CAS  PubMed  Google Scholar 

  • Dunon D, Piali L, Imhof BA (1996) To stick or not to stick: the new leukocyte homing paradigm. Curr Opin Cell Biol 8:714–723

    CAS  PubMed  Google Scholar 

  • Erladsen SL, Hasslen SR, Nelson RD (1993) Detection and spatial distribution of the β2 integrin (Mac-1) and L-selectin (LECAM-1) adherence receptors on human neutrophils by high-resolution field emission SEM. J Histochem Cytochem 41:327–333

    CAS  PubMed  Google Scholar 

  • Ley K, Tedder TF (1995) Leukocyte interactions with vascular endothelium. New insights into selectin-mediated attachment and rolling. J Immunol 155:525–528

    CAS  PubMed  Google Scholar 

  • Poncelet P, Carayon P (1985) Cytofluorimetric quantification of cell-surface antigens by indirect immunofluorescence using monoclonal antibodies. J Immunol Methods 85:65–74

    Article  CAS  PubMed  Google Scholar 

  • Poncelet P, George F, Papa S, Lanza F (1996) Quantification of hematopoietic cell antigens in flow cytometry. Eur J Histochem Cytochem 40:15–32

    Google Scholar 

  • Rochon YP, Kavanagh TJ, Harlan JM (2000) Analysis of integrin (CD11b/CD18) movement during neutrophil adhesion and migration on endothelial cells. J Microsc 197:15–24

    Article  CAS  PubMed  Google Scholar 

  • Scalletar BA, Swedlow JR, Sedat JW, Agard DA (1996) Dispersion, aberration and deconvolution in multi-wavelength fluorescence images. J Microsc 182:50–59

    PubMed  Google Scholar 

  • Shao JY, Beall HPT, Hochmuth RM (1998) Static and dynamic length of neutrophil microvilli. Proc Natl Acad Sci USA 95:6797–6802

    Article  CAS  PubMed  Google Scholar 

  • Shaw PJ (1995) Comparison between confocal and wide-field imaging in 3-D microscopy. In: Pawley J B (ed) Handbook of biological confocal microscopy. Plenum Press, New York, pp 373–387

  • Zhu C (2000) Kinetics and mechanics of cell adhesion. J Biomech 33:23–33

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

P.R. gratefully acknowledges the financial support from the Grant Agency of the Czech Republic (grant no. 305/01/1605).

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Authors and Affiliations

  1. Institute of Hydrodynamics, Academy of Sciences of the Czech Republic, Pod Patankou 5, 166 12 , Prague, Czech Republic

    P. Riha

  2. Cell and Tissue Mechanics and Engineering, LEMTA UMR-CNRS 7563, Faculty of Medicine, 54505 , Vandoeuvre les Nancy, France

    V. Labrador, P. Riha, S. Muller, D. Dumas, X. Wang & J. F. Stoltz

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  1. V. Labrador
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  2. P. Riha
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  3. S. Muller
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  4. D. Dumas
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  5. X. Wang
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  6. J. F. Stoltz
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Correspondence to P. Riha.

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Labrador, V., Riha, P., Muller, S. et al. The strength of integrin binding between neutrophils and endothelial cells. Eur Biophys J 32, 684–688 (2003). https://doi.org/10.1007/s00249-003-0329-4

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  • DOI: https://doi.org/10.1007/s00249-003-0329-4

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