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Microbial Ecology

, Volume 39, Issue 1, pp 49–55 | Cite as

Natural Endophytic Occurrence of Acetobacter diazotrophicus in Pineapple Plants

  • A.  Tapia-Hernández
  • M.R.  Bustillos-Cristales
  • T.  Jiménez-Salgado
  • J.  Caballero-Mellado
  • L.E.  Fuentes-Ramírez

Abstract

The presence of endophytic Acetobacter diazotrophicus was tested for pineapple plants (Ananas comosus [L.] Merr.) grown in the field. Diazotrophic bacteria were isolated from the inner tissues of surface sterilized roots, stems, and leaves of pineapple plants. Phenotypic tests permitted the selection of presumptive nitrogen-fixing A. diazotrophicus isolates. Restriction fragment length polymorphisms (RFLPs) of small subunit (SSU) rDNA using total DNA digested with endonuclease SphI and with endonuclease NcoI, hybridizations of RNA with an A. diazotrophicus large subunit (LSU) rRNA specific probe, as well as patterns in denaturing protein electrophoresis (SDS-PAGE) and multilocus enzyme tests allowed the identification of A. diazotrophicus isolates. High frequencies of isolation were obtained from propagative buds that had not been nitrogen-fertilized, and lower frequencies from 3-month-old plants that had been nitrogen-fertilized. No isolates were recovered from 5- to 7-month-old nitrogen-fertilized plants. All the A. diazotrophicus isolates recovered from pineapple plants belonged to the multilocus genotype which shows the most extensive distribution among all host species previously analyzed.

Keywords

Restriction Fragment Length Polymorphism Restriction Fragment Fragment Length Polymorphism Host Species Large Subunit 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 2000

Authors and Affiliations

  • A.  Tapia-Hernández
    • 1
  • M.R.  Bustillos-Cristales
    • 2
  • T.  Jiménez-Salgado
    • 1
  • J.  Caballero-Mellado
    • 2
  • L.E.  Fuentes-Ramírez
    • 1
  1. 1.Laboratorio de Microbiología de Suelos, Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Complejo de Ciencias, Edif. 76, Ciudad Universitaria, Puebla, Pue., MéxicoMX
  2. 2.Programa de Ecología Microbiana y Molecular, Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Apdo. Postal 565-A, Cuernavaca, Mor., MéxicoMX

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