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Structure and colonization dynamics of epiphytic bacterial communities and of selected component strains on tomato (Lycopersicon esculentum) leaves

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Abstract

The sizes and compositions of bacterial populations found on leaves of greenhouse and field grown tomato plants were studied by dilution plating, fatty acid methyl ester analysis (FAME), and BIOLOG plates of isolates in pure cultures. In the greenhouse, overhead-irrigated plants sustained higher microbial populations (up to 105 cfu g−1) than soil-irrigated plants (103 cfu g−1). Strains isolated from overhead-irrigated plants grown in a vegetable garden (n=216) and from greenhouse-grown plants (n=114) were subjected to FAME analysis. Similarly, strains from soil-irrigated field-grown plants (n=83) were identified using BIOLOG plates. In each case, populations were dominated by a few genera. When concentrated phyllosphere washes (CPW) were sprayed on greenhouse-grown, soil-irrigated plants, leaf bacterial populations of more than 105 CFU g−1 were sustained for 4 days; sterile buffer-sprayed leaves sustained less than 104 CFU g−1. No significant enrichment of any strain isolated from the sprayed leaves could be detected by FAME identification of randomly selected colonies. However, when recurring leaf saprophytic species (both Gram-positive and Gram-negative) isolated from these experiments and from plants grown outdoors were tested for epiphytic colonization under stressful conditions, all could still be detected at various levels up to 4 days after inoculation, indicating differential epiphytic fitness. The non-epiphytic bacteriaEscherichia coli andAzospirillum brasilense disappeared from the leaf surface within the same experimental period.

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Authors and Affiliations

  1. Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O.B. 12, 76100, Rehovot, Israel

    Edouard J. Jurkevitch & G. Shapira

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  1. Edouard J. Jurkevitch
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  2. G. Shapira
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Correspondence to Edouard J. Jurkevitch.

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Jurkevitch, E.J., Shapira, G. Structure and colonization dynamics of epiphytic bacterial communities and of selected component strains on tomato (Lycopersicon esculentum) leaves. Microb Ecol 40, 300–308 (2000). https://doi.org/10.1007/s002480000023

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