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Mediative Mechanism of Freezing/Thawing on Greenhouse Gas Emissions in an Inland Saline-Alkaline Wetland: a Metagenomic Analysis

  • Environmental Microbiology
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A Correction to this article was published on 16 March 2023

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Abstract

Inland saline-alkaline wetlands distributed in the mid-high latitude have repeatedly experienced freezing and thawing. However, the response of greenhouse gas (GHG) emission and microbially-mediated carbon and nitrogen cycle to freezing and thawing remains unclear. We monitored the GHG flux in an inland saline-alkaline wetland and found that, compared with the growth period, the average CO2 flux decreased from 171.99 to 76.61–80.71 mg/(m2‧h), the average CH4 flux decreased from 10.72 to 1.96–3.94 mg/(m2‧h), and the average N2O flux decreased from 56.17 to − 27.14 to − 20.70 μg/(m2‧h). Freezing and thawing significantly decreased the relative abundance of functional genes involved in carbon and nitrogen cycles. The aceticlastic methanogenic pathway was the main methanogenic pathway, whereas the Candidatus Methylomirabilis oxyfera was the most abundant methane oxidizer in the wetland. Ammonia-oxidizing archaea and denitrifier belonging to proteobacteria was the major microbial N2O source, while bacteria within clade II nosZ was the major microbial N2O sink. Freezing and thawing reduced the relative abundance of these genes, leading to a decrease in GHG flux.

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Data Availability

All the raw sequences in this study can be found in the National Center for Biotechnology Information Sequence Read Archive under BioProject PRJNA773918 with the accession number SAMN22543995–SAMN22544000.

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Funding

This research was supported by the National Natural Science Foundation of China (No. 31470543) and the Key Technology Research and Development Program of Henan (Nos. 222102320379 and 222102320197).

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Authors and Affiliations

  1. Collaborative Innovation Center of Energy Conservation & Emission Reduction and Sustainable Urban-Rural Development in Beijing, Beijing University of Civil Engineering and Architecture, Beijing, 100044, China

    Yupeng Zhang, Hong Liang & Dawen Gao

  2. College of Resources and Environmental Sciences, Henan Agricultural University, No.63 Agricultural Road, Zhengzhou, 450002, China

    Yupeng Zhang

  3. College of Life Sciences, Henan Agricultural University, No.63 Agricultural Road, Zhengzhou, 450002, China

    Fengqin Liu

  4. Centre for Urban Environmental Remedeation, Beijing University of Civil Engineering and Architecture, Beijing, 100044, China

    Hong Liang & Dawen Gao

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Yupeng Zhang: Conceptualization, investigation, formal analysis, visualization, and writing—original draft. Fengqin Liu: Resources, project administration, review and editing, and data curation. Hong Liang: Writing—review and editing, supervision, and funding acquisition. Dawen Gao: Investigation and visualization.

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Zhang, Y., Liu, F., Liang, H. et al. Mediative Mechanism of Freezing/Thawing on Greenhouse Gas Emissions in an Inland Saline-Alkaline Wetland: a Metagenomic Analysis. Microb Ecol 86, 985–996 (2023). https://doi.org/10.1007/s00248-022-02165-8

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