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Prokaryotic, Microeukaryotic, and Fungal Composition in a Long-Term Polychlorinated Biphenyl-Contaminated Brownfield

  • Soil Microbiology
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Abstract

Polychlorinated biphenyls (PCBs) are recognized as persistent organic pollutants and accumulate in organisms, soils, waters, and sediments, causing major health and ecological perturbations. Literature reported PCB bio-transformation by fungi and bacteria in vitro, but data about the in situ impact of those compounds on microbial communities remained scarce while being useful to guide biotransformation assays. The present work investigated for the first time microbial diversity from the three-domains-of-life in a long-term contaminated brownfield (a former factory land). Soil samples were ranked according to their PCB concentrations, and a significant increase in abundance was shown according to increased concentrations. Microbial communities structure showed a segregation from the least to the most PCB-polluted samples. Among the identified microorganisms, Bacteria belonging to Gammaproteobacteria class, as well as Fungi affiliated to Saccharomycetes class or Pleurotaceae family, including some species known to transform some PCBs were abundantly retrieved in the highly polluted soil samples.

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Data Availability

All data that support findings of this study have been deposited in European Nucleotide Archive (https://www.ebi.ac.uk/ena/browser/view/PRJEB46614) under reference PRJEB46614 for 16S rRNA dataset, PRJEB46555 and PRJEB46556 for ITS regions and eukaryotic 18S rRNA genes, respectively.

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Acknowledgements

We would like to thank Jean Thioulouse (LBBE—UMR CNRS 5558—Villeurbanne) for helpful discussions.

Funding

This work was supported by the French MITI-CNRS [urban ecosystem—AAP 2019], EC2CO – CNRS [CNRS INSU—AAP 2020] and French Auvergne-Rhône-Alpes AURA Region [Pack Ambition Recherche 2021] programs. F. Maucourt PhD was supported by a Research and Technology French National association ANRT and Envisol fellowship.

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Author notes

  1. Mylène Hugoni

    Present address: Univ Lyon, INSA Lyon, CNRS, UMR 5240 Microbiologie Adaptation Et Pathogénie, 69621, Villeurbanne, France

  2. Mylène Hugoni and Laurence Fraissinet-Tachet contributed equally to this work.

Authors and Affiliations

  1. Univ Lyon, Université Claude Bernard Lyon 1, CNRS, INRAE, VetAgro Sup, UMR Ecologie Microbienne, 69622, Villeurbanne, France

    Flavien Maucourt, Sonia Czarnes, Delphine Melayah, David Chapulliot, Laurent Vallon, Mylène Hugoni & Laurence Fraissinet-Tachet

  2. ENVISOL, 2-4 Rue Hector Berlioz, 38110, La Tour du Pin, France

    Flavien Maucourt & Gaël Plassart

  3. Université de Lorraine, CNRS, LIEC, 54000, Nancy, France

    Aurélie Cébron

  4. Université de Bordeaux, CNRS, EPOC, 33405, Bordeaux, France

    Hélène Budzinski, Karyn Le Menach & Laurent Peluhet

  5. Univ Lyon, INSA Lyon, CNRS, UMR 5240 Microbiologie Adaptation Et Pathogénie, 69621, Villeurbanne, France

    Delphine Melayah

  6. Institut Universitaire de France (IUF), Paris, France

    Mylène Hugoni

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  1. Flavien Maucourt
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Contributions

All authors contributed to the study conception and design. Material preparation and data collection were performed by F. Maucourt, A. Cébron, H. Budzinski, K. Le Ménach, L. Peluhet, D. Chapulliot, L. Vallon, S. Czarnes, and M. Hugoni. Data analyses were performed by F. Maucourt, A. Cébron, M. Hugoni, and L. Fraissinet-Tachet. The first draft of the manuscript was written by F. Maucourt, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Laurence Fraissinet-Tachet.

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Maucourt, F., Cébron, A., Budzinski, H. et al. Prokaryotic, Microeukaryotic, and Fungal Composition in a Long-Term Polychlorinated Biphenyl-Contaminated Brownfield. Microb Ecol 86, 1696–1708 (2023). https://doi.org/10.1007/s00248-022-02161-y

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