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The Role of Diversity in Mediating Microbiota Structural and Functional Differences in Two Sympatric Species of Abalone Under Stressed Withering Syndrome Conditions

  • Invertebrate Microbiology
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Abstract

Withering syndrome (WS) is a gastro-intestinal (GI) infectious disease likely affecting all abalone species worldwide. Structural and functional changes in abalone GI microbiotas under WS-stressed conditions remain poorly investigated. It is unclear if interspecific microbiota differences, such as the presence of certain microbes, their abundance, and functional capabilities, may be involved in the occurrence of this disease. Bacterial microbiotas of healthy Haliotis fulgens and Haliotis corrugata are mainly composed by Tenericutes, Proteobacteria, Fusobacteria, and Spirochaetes. We previously reported species-specific structural and functional profiles of those communities and suggested that they are of consequence to the different susceptibility of each species to WS. Here, we address this question by comparing the structure and function of healthy and dysbiotic microbiota through 454 pyrosequencing and PICRUSt 2, respectively. Our findings suggest that the extent to which WS-stressed conditions may explain structural and functional differences in GI microbiota is contingent on the microbiota diversity itself. Indeed, microbiota differences between stressed and healthy abalone were marginal in the more complex bacterial communities of H. corrugata, in which no significant structural or functional changes were detected. Conversely, significant structural changes were observed in the less complex bacterial microbiota of H. fulgens. Moreover, structural alterations led to a significant downregulation of some metabolic activities conducted by GI bacteria. Accordingly, results suggest that gastro-intestinal bacterial diversity appears to be related with both the health of abalone and the etiology of WS.

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Availability of Data and Materials

Raw reads were deposited in the National Center for Biotechnology Information (NCBI; BioProject: PRJNA494699, accession number: SRR7969002).

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Acknowledgements

We are grateful to Dr. W. Kelley Thomas for providing us with the possibility of running bioinformatics pipelines using the server of the Research Computing Center of the University of New Hampshire and Andrea Lievana-MacTavish for English language editing.

Funding

This study was supported by SAGARPA-CONACYT [grant number 2011-C01-163322], UC Mexus-CONACYT [grant number CN-14–14], and the Fund for Scientific Research and Technological Development of CICESE [grant number FID-2012–01]. The first author received a graduate fellowship from CONACYT to support his Ph.D. research in Marine Ecology at the Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE). sagarpa-conacyt,2011-CO1-163322,Axayácatl Rocha-Olivares,uc mexus-conacyt,CN-14–14,Axayácatl Rocha-Olivares,cicese,Fund for Scientific Research,Axayácatl Rocha-Olivares,Technological Development FID-2012–01,Axayácatl Rocha-Olivares

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Authors and Affiliations

  1. Department of Biological Oceanography, CICESE, Carretera Tijuana-Ensenada km 3918, 22860, Ensenada, Baja CA, Mexico

    Francesco Cicala, José Alejandro Cisterna-Céliz & Axayácatl Rocha-Olivares

  2. Department of Biomedical Innovation, CICESE, Carretera Tijuana-Ensenada km 3918, 22860, Ensenada, Baja CA, Mexico

    Francesco Cicala

  3. National Council of Scientific and Technical Research (CONICET), Av. Rivadavia 1917, C1033AAJ, Buenos Aires, Argentina

    Marcos Paolinelli

  4. National Institute of Agricultural Technology INTA EEA Mendoza, San Martin 3853, Luján de Cuyo, 5507, Mendoza, Argentina

    Marcos Paolinelli

  5. Bodega Marine Laboratory, University of California at Davis, P.O. Box 247, Bodega Bay, CA, USA

    James D. Moore

  6. Hubbard Center for Genome Studies, University of New Hampshire, 35 Colovos Rd, Durham, NH, 03824, USA

    Joseph Sevigny

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  1. Francesco Cicala
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  2. José Alejandro Cisterna-Céliz
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  3. Marcos Paolinelli
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  4. James D. Moore
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  6. Axayácatl Rocha-Olivares
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Contributions

Experimental design: Francesco Cicala, James D. Moore, and Axayácatl Rocha-Olivares. Data collection and preparation: Francesco Cicala. Bioinformatics analysis: Francesco Cicala, José Alejandro Cisterna-Céliz, Marcos Paolinelli, and Joseph Sevigny. Funding acquisition: Axayácatl Rocha-Olivares and James D. Moore. The first draft of the manuscript was written by Francesco Cicala and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Axayácatl Rocha-Olivares.

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Cicala, F., Cisterna-Céliz, J.A., Paolinelli, M. et al. The Role of Diversity in Mediating Microbiota Structural and Functional Differences in Two Sympatric Species of Abalone Under Stressed Withering Syndrome Conditions. Microb Ecol 85, 277–287 (2023). https://doi.org/10.1007/s00248-022-01970-5

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