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Short-Term Dynamics of Bdellovibrio and Like Organisms in Lake Geneva in Response to a Simulated Climatic Extreme Event

Abstract

The short time-scale dynamics of three families of Bdellovibrio and like organisms (i.e. Bdellovibrionaceae, Peredibacteraceae, and Bacteriovoracaceae) were studied on the surface waters of Lake Geneva in summer. Using mesocosms deployed nearshore in July 2019, we simulated an extreme climatic event (an input of carbon from the watershed in response to runoff from the catchment, light reduction, and mixing in response to stormy conditions) and aimed to study the impact of both abiotic and biotic factors on their dynamics. The three families of Bdellovibrio and like organisms (BALOs) showed different dynamics during the experiment. Peredibacteraceae was the most abundant group, whereas Bacteriovoracaceae was the least abundant. Compared with the other two families, the abundance of Bdellovibrionaceae did not fluctuate, remaining relatively stable over time. Environmental variables only partially explained the dynamics of these families; in particular, temperature, pH, and chloride concentrations were positively correlated with Bacteriovoracaceae, Bdellovibrionaceae, and Peredibacteraceae abundance, respectively. Prokaryote-like particles (PLPs), such as those with high DNA content (HDNA), were strongly and positively correlated with Peredibacteraceae and Bacteriovoracaceae. In contrast, no relationships were found between Bdellovibrionaceae and PLP abundance, nor between the virus-like particles (VLPs) and the different BALOs. Overall, the experiment revealed that predation was stable in the face of the simulated climatic events. In addition, we observed that Peredibacteraceae and Bacteriovoracaceae share common traits, while Bdellovibrionaceae seems to constitute a distinct category.

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Data Availability

All data can be made available. It is noteworthy that two data papers have been published on the MESOLAC data (https://doi.org/10.1016/j.dib.2020.106255 and https://doi.org/10.1016/j.dib.2021.107150).

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Acknowledgements

The authors would like to thank all those who participated in the MESOLAC project: Laurent Espinat, Clementine Gallot, Pascal Perney, Jean-Christophe Hustache, Philippe Quetin, Viet Tran-Khac, Laura Crepin, and Mathilde Chevallay. We also thank Cécilia Barouillet and Teofana Chonova for their statistical assistance. Finally, professional scuba divers (Jean-Marc Bel, Stéphanie Kocca, Stéphan Jacquet and Eric Mocelin) are gratefully acknowledged for their help in anchoring the eco-friendly (e.g. ecological mooring) mesocosms. We would like to thank Editage (www.editage.com) for English language editing.

Funding

JE (PhD grant) was funded by INRAE and University Savoie-Mont Blanc. AJ (Master grant) was funded by INRAE. The MESOLAC project was funded by INRAE and ANAEE France.

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JE participated to data analysis and writing. AJ helped to analyse the data. SR and ID co-organised the MESOLAC project with SJ. SJ co-organised the MESOLAC project with SR and ID, planned this study, participated to writing, and submitted the manuscript.

Corresponding author

Correspondence to S. Jacquet.

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Ezzedine, J.A., Janicot, A., Rasconi, S. et al. Short-Term Dynamics of Bdellovibrio and Like Organisms in Lake Geneva in Response to a Simulated Climatic Extreme Event. Microb Ecol (2021). https://doi.org/10.1007/s00248-021-01875-9

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Keywords

  • Bdellovibrio and like organisms
  • Dynamics
  • Prokaryotes
  • Lake Geneva
  • Ecological stress