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Year, Location, and Variety Impact on Grape-, Soil-, and Leaf-Associated Fungal Microbiota of Arkansas-Grown Table Grapes

Microbial Ecology volume 82, pages 73–86 (2021)Cite this article

Abstract

With the recent advancement of next-generation sequencing methods, there has been an increase in studies on identification of vineyard microbiota, winery-associated microbiota, and microbiota in wine fermentation. However, there have been few studies investigating the fungal microbiota of table grapes which present distinct spoilage and food safety challenges. The aims of this study were to identify and compare the impact of year, variety, and vineyard location on grape, leaf, and soil fungal communities of two varieties of table grapes, Faith and Gratitude, grown in two open-air vineyards and one high tunnel vineyard. The grape, leaf, and soil mycobiota were analyzed using high throughput amplicon sequencing of the ITS region. The sampling year and location of table grapes had an impact on grape, leaf, and soil mycobiota. Fungal diversity of grape, leaf, and soil was greater in 2017 than in 2016. Grape and leaf samples presented strong similarities in fungal communities with abundance of Sporidiobolaceae and Filobasidium in two vineyards and Cladosporium in another one. The high tunnel structure had distinct grape and leaf fungal communities compared to the two other vineyard locations. Mortierella was the predominant genus (27%) in soil samples for the three locations; however, genera of lower abundance varied between locations. These results provide extensive description of fungal communities in less-studied table grape vineyards and high tunnels, providing useful insight of potential threats and preventive strategies to help improve the production and marketability of table grapes.

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Data Availability

The datasets generated and/or analyzed during the current study are available from the corresponding author upon reasonable request.

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Acknowledgements

The authors would like to thank the vineyard managers and winemakers from Post Vineyards and Winery, the University of Arkansas System Division of Agriculture Fruit Research Station and Research Extension Center for their generous contribution of table grape, leaf, and soil samples.

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Affiliations

  1. Food Science Department, University of Arkansas, Fayetteville, AR, USA

    Natacha Cureau, Renee Threlfall, Daya Marasini, Laura Lavefve & Franck Carbonero

  2. Crop, Soil, and Environmental Sciences Department, University of Arkansas, Fayetteville, AR, USA

    Mary Savin

  3. Nutrition and Exercise Physiology, Elson S. Floyd College of Medicine, Washington State University, Spokane, WA, USA

    Franck Carbonero

  4. School of Food Science, Washington State University, Spokane, WA, USA

    Franck Carbonero

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  1. Natacha Cureau
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  2. Renee Threlfall
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  4. Daya Marasini
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  5. Laura Lavefve
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Contributions

Natacha Cureau, Franck Carbonero, Renee Threlfall, and Mary Savin contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Natacha Cureau, Laura Lavefve, and Daya Marasini. The first draft of the manuscript was written by Natacha Cureau, Renee Threlfall, and Mary Savin and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Franck Carbonero.

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Cureau, N., Threlfall, R., Savin, M. et al. Year, Location, and Variety Impact on Grape-, Soil-, and Leaf-Associated Fungal Microbiota of Arkansas-Grown Table Grapes. Microb Ecol 82, 73–86 (2021). https://doi.org/10.1007/s00248-021-01698-8

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Keywords

  • Fungi
  • Vineyards
  • DNA high-throughput sequencing
  • High tunnels
  • Faith
  • Gratitude