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Co-association of Two nir Denitrifiers Under the Influence of Emergent Macrophytes

  • Environmental Microbiology
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Abstract

Diverse microorganisms perform similar metabolic process in biogeochemical cycles, whereas they are found of highly genomic differentiation. Biotic interactions should be considered in any community survey of these functional groups, as they contribute to community assembly and ultimately alter ecosystem properties. Current knowledge has mainly been achieved based on functional community characterized by a single gene using co-occurrence network analysis. Biotic interactions between functionally equivalent microorganisms, however, have received much less attention. Herein, we propose the nirK- and nirS-type denitrifier communities represented by these two nitrite reductase (nir)–encoding genes, as model communities to investigate the potential interactions of two nir denitrifiers. We evaluated co-occurrence patterns and co-association network structures of nir denitrifier community from an emergent macrophyte-dominated riparian zone of highly active denitrification in Lake Taihu, China. We found a more segregated pattern in combined nir communities than in individual communities. Network analyses revealed a modularized structure of associating nir denitrifiers. An increased proportion of negative associations among combined communities relative to those of individual communities indicated potential interspecific competition between nirK and nirS denitrifiers. pH and NH4+-N were the most important factors driving co-occurrence and mutual exclusion between nirK and nirS denitrifiers. We also showed the topological importance of nirK denitrifiers acting as module hubs for constructing entire association networks. We revealed previously unexplored co-association relationships between nirK and nirS denitrifiers, which were previously neglected in network analyses of individual communities. Using nir denitrifier community as a model, these findings would be helpful for us to understand the biotic interactions and mechanisms underlying how functional groups co-exist in performing biogeochemical cycles.

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Funding

This work was supported by the Science & Technology Basic Resources Investigation Program of China (2017FY100300), the National Natural Science Foundation of China (31730013, 41621002, 41871096, and 31971478), the Fundamental Research Funds for the Central Universities (2018B43414 and 2019B17814), and the China Postdoctoral Science Foundation (2018M642147).

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Authors and Affiliations

  1. State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, School of Earth Sciences and Engineering, Hohai University, Nanjing, 210098, China

    Rui Huang, Dayong Zhao, Bin Yong & Zhongbo Yu

  2. State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, 210008, China

    Jin Zeng

Authors
  1. Rui Huang
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  2. Jin Zeng
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  3. Dayong Zhao
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  4. Bin Yong
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  5. Zhongbo Yu
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Contributions

J.Z. and D.Z. designed and supported the study. R.H. carried out the experiment and analyzed the data. R.H. prepared the first draft. D.Z. provided the improvements for the final manuscript. B.Y. and Z.Y. carried out the lab work and contributed to commenting and revising. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Jin Zeng.

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The authors declare that they have no conflict of interest.

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Huang, R., Zeng, J., Zhao, D. et al. Co-association of Two nir Denitrifiers Under the Influence of Emergent Macrophytes. Microb Ecol 80, 809–821 (2020). https://doi.org/10.1007/s00248-020-01545-2

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  • DOI: https://doi.org/10.1007/s00248-020-01545-2

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