Rain-Fed Granite Rock Basins Accumulate a High Diversity of Dormant Microbial Eukaryotes

Microbial Ecology volume 79, pages882–897(2020)Cite this article

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Abstract

Rain fed granite rock basins are ancient geological landforms of worldwide distribution and structural simplicity. They support habitats that can switch quickly from terrestrial to aquatic along the year. Diversity of animals and plants, and the connexion between communities in different basins have been widely explored in these habitats, but hardly any research has been carried out on microorganisms. The aim of this study is to provide the first insights on the diversity of eukaryotic microbial communities from these environments. Due to the ephemeral nature of these aquatic environments, we predict that the granitic basins should host a high proportion of dormant microeukaryotes. Based on an environmental DNA diversity survey, we reveal diverse communities with representatives of all major eukaryotic taxonomic supergroups, mainly composed of a diverse pool of low abundance OTUs. Basin communities were very distinctive, with alpha and beta diversity patterns non-related to basin size or spatial distance respectively. Dissimilarity between basins was mainly characterised by turnover of OTUs. The strong microbial eukaryotic heterogeneity observed among the basins may be explained by a complex combination of deterministic factors (diverging environment in the basins), spatial constraints, and randomness including founder effects. Most interestingly, communities contain organisms that cannot coexist at the same time because of incompatible metabolic requirements, thus suggesting the existence of a pool of dormant organisms whose activity varies along with the changing environment. These organisms accumulate in the pools, which turns granitic rock into high biodiversity microbial islands whose conservation and study deserve further attention.

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Change history

  • 23 December 2019

    The original version of this article contained an erratum of omission in the Acknowledgments section.

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Acknowledgements

Permits to collect samples and facilities provided by The Parque Nacional Sierra de Guadarrama are gratefully acknowledged.

Funding

This study was funded by Ministerio de Economía y Competitividad (MINECO-Spain), Project MICROEPICS (Ref: CGL2013-40851-P/ BOS 2014-2018; PI: MM-C). EL was funded by a project “Atraccion de talento investigador” by the Consejería de Educación, Juventud y Deporte, Comunidad de Madrid (Spain) 2017-T1/AMB-5210 and by a grant from the Swiss National Foundation for Research SNF 31003A_143960.

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Affiliations

  1. Departamento de Genética, Fisiología y Microbiología. Facultad de Ciencias Biológicas, Universidad Complutense de Madrid (UCM), C/ José Antonio Novais 12, 28040, Madrid, Spain

    Ismael Velasco-González & Mercedes Martín-Cereceda

  2. Departamento de Biodiversidad, Ecología y Evolución. Facultad de Ciencias Biológicas, UCM, Madrid, Spain

    Abel Sanchez-Jimenez, Antonio Murciano & Sergio Díez-Hermano

  3. Laboratory of Soil Biodiversity, Institute of Biology, University of Neuchâtel, Rue Emile-Argand 11, CH-2000, Neuchâtel, Switzerland

    David Singer

  4. Department of Zoology, Institute of Biosciences, University of São Paulo, São Paulo, 05508-090, Brazil

    David Singer

  5. Real Jardín Botánico, CSIC Plaza de Murillo 2, 28014, Madrid, Spain

    Enrique Lara

Authors
  1. Ismael Velasco-González
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  2. Abel Sanchez-Jimenez
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  3. David Singer
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  4. Antonio Murciano
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  5. Sergio Díez-Hermano
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  6. Enrique Lara
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  7. Mercedes Martín-Cereceda
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Corresponding author

Correspondence to Mercedes Martín-Cereceda.

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Enrique Lara and Mercedes Martin-Cereceda share last co-authorship

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Velasco-González, I., Sanchez-Jimenez, A., Singer, D. et al. Rain-Fed Granite Rock Basins Accumulate a High Diversity of Dormant Microbial Eukaryotes. Microb Ecol 79, 882–897 (2020). https://doi.org/10.1007/s00248-019-01463-y

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Keywords

  • Granite rock basins
  • Microbial reservoirs
  • Protists
  • Fungi
  • Dormancy
  • Conservation