Phosphorus Input Alters the Assembly of Rice (Oryza sativa L.) Root-Associated Communities

Abstract

Rice root–associated microbial community play an important role in plant nutrient acquisition, biomass production, and stress tolerance. Herein, root-associated community assembly was investigated under different phosphate input levels in phosphorus (P)-deficient paddy soil. Rice was grown in a long-term P-depleted paddy soil with 0 (P0), 50 (PL), or 200 (PH) mg P2O5 kg−1 application. DNA from root endophytes was isolated after 46 days, and PCR amplicons from archaea, bacteria, and fungi were sequenced by an Illumina Miseq PE300 platform, respectively. P application had no significant effect on rice root endophytic archaea, which were dominated by ammonia-oxidizing Candidatus Nitrososphaera. By contrast, rice root endophytic community structure of the bacteria and fungi was affected by soil P. Low P input increased endophytic bacterial diversity, whereas high P input increased rhizosphere fungi diversity. Bacillus and Pleosporales, associated with phosphate solubilization and P uptake, dominated in P0 and PH treatments, and Pseudomonas were more abundant in the PL treatment than in the P0 and PH treatments. Co-occurrence network analysis revealed a close interaction between endophytic bacteria and fungi. Soil P application affected both the rice root endosphere and soil rhizosphere microbial community and interaction between rice root endophytic bacteria, and fungi, especially species related to P cycling.

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Acknowledgments

We appreciate Prof. Wenxue Wei (the Institute of Subtropical Agriculture, CAS) for the assistance with soil sampling and Dr. Jun Xiong (Fujian Agriculture and Forestry University) for the help with endophytic DNA extraction.

Funding

This work was supported by the National Natural Science Foundation of China (41525002, 41773079) and the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB15020303).

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Correspondence to Huaiying Yao.

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Long, XE., Yao, H. Phosphorus Input Alters the Assembly of Rice (Oryza sativa L.) Root-Associated Communities. Microb Ecol 79, 357–366 (2020). https://doi.org/10.1007/s00248-019-01407-6

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Keywords

  • Network analysis,
  • Phosphate input,
  • Rice (Oryza sativa L.),
  • Root associated,
  • Endophytic community