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Assessing the Microbiota of Black Soldier Fly Larvae (Hermetia illucens) Reared on Organic Waste Streams on Four Different Locations at Laboratory and Large Scale

  • E. Wynants
  • L. Frooninckx
  • S. Crauwels
  • C. Verreth
  • J. De Smet
  • C. Sandrock
  • J. Wohlfahrt
  • J. Van Schelt
  • S. Depraetere
  • B. Lievens
  • S. Van Miert
  • J. Claes
  • L. Van Campenhout
Environmental Microbiology

Abstract

This study aimed to gain insight into the microbial quality, safety and bacterial community composition of black soldier fly larvae (Hermetia illucens) reared at different facilities on a variety of organic waste streams. For seven rearing cycles, both on laboratory-scale and in large-scale facilities at several locations, the microbiota of the larvae was studied. Also samples of the substrate used and the residue (= leftover substrate after rearing, existing of non-consumed substrate, exuviae and faeces) were investigated. Depending on the sample, it was subjected to plate counting, Illumina Miseq sequencing and/or detection of specific food pathogens. The results revealed that the substrates applied at the various locations differed substantially in microbial numbers as well as in the bacterial community composition. Furthermore, little similarity was observed between the microbiota of the substrate and that of the larvae reared on that substrate. Despite substantial differences between the microbiota of larvae reared at several locations, 48 species-level operational taxonomic units (OTUs) were shared by all larvae, among which most belonged to the phyla Firmicutes and Proteobacteria. Although the substrate is assumed to be an important source of bacteria, our results suggest that a variety of supposedly interacting factors-both abiotic and biotic-are likely to affect the microbiota in the larvae. In some larvae and/or residue samples, potential foodborne pathogens such as Salmonella and Bacillus cereus were detected, emphasising that decontamination technologies are required when the larvae are used in feed, just as for other feed ingredients, or eventually in food.

Keywords

Hermetia illucens Microbiota Laboratory scale Industrial scale High-throughput sequencing Plate counts 

Notes

Funding

The research that yielded these results was funded by the Belgian Federal Public Service of Health, Food Chain Safety and Environment through the contract RT 15/9 EDINCO.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

All applicable international, national and/or institutional guidelines for the care and use of animals were followed.

Supplementary material

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • E. Wynants
    • 1
  • L. Frooninckx
    • 2
  • S. Crauwels
    • 3
  • C. Verreth
    • 3
  • J. De Smet
    • 1
  • C. Sandrock
    • 4
  • J. Wohlfahrt
    • 4
  • J. Van Schelt
    • 5
  • S. Depraetere
    • 6
  • B. Lievens
    • 3
  • S. Van Miert
    • 2
  • J. Claes
    • 1
  • L. Van Campenhout
    • 1
  1. 1.Department of Microbial and Molecular Systems (M2S), Lab4FoodKU LeuvenGeelBelgium
  2. 2.Thomas More University of Applied Sciences, RADIUSGeelBelgium
  3. 3.Department of Microbial and Molecular Systems (M2S), Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM)KU LeuvenSint-Katelijne-WaverBelgium
  4. 4.Research Institute of Organic Agriculture (FiBL)FrickSwitzerland
  5. 5.Koppert Biological SystemsBerkel en RodenrijsThe Netherlands
  6. 6.MillibeterTurnhoutBelgium

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