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Experimental Testing of Dispersal Limitation in Soil Bacterial Communities with a Propagule Addition Approach

Microbial Ecology volume 77, pages 905–912 (2019)Cite this article

Abstract

The role of dispersal in the assembly of microbial communities remains contentious. This study tested the importance of dispersal limitation for the structuring of local soil bacterial communities using an experimental approach of propagule addition. Microbes extracted from soil pooled from samples collected at 20 localities across ~ 400 km in a temperate steppe were added to microcosms of local soils at three sites; the microcosms were then incubated in situ for 3 months. We then assessed the composition and diversity of bacterial taxa in the soils using 16S rRNA gene amplicon sequencing. The addition of the regional microbial pool did not cause significant changes in the overall composition or diversity of the total bacterial community, although a very small number of individual taxa may have been affected by the addition treatment. Our results suggest a negligible role of dispersal limitation in structuring soil bacterial communities in our study area.

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Funding

This study was funded by the National Natural Science Foundation of China (31700434, 31725006, and 31670376) and the 111 project (B13008). The 16S rRNA gene sequence data were deposited in the NCBI Sequence Read Archive under accession number SRP057046.

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Authors and Affiliations

  1. College of Life Sciences, Shanxi Normal University, Linfen, 041004, Shanxi, China

    Fen-Guo Zhang

  2. Department of Life Sciences, Imperial College London, Ascot, Berkshire, SL5 7PY, UK

    Thomas Bell

  3. State Key Laboratory of Earth Surface Processes and Resource Ecology and MOE Key Laboratory for Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, Beijing, 100875, China

    Quan-Guo Zhang

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Correspondence to Quan-Guo Zhang.

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Zhang, FG., Bell, T. & Zhang, QG. Experimental Testing of Dispersal Limitation in Soil Bacterial Communities with a Propagule Addition Approach. Microb Ecol 77, 905–912 (2019). https://doi.org/10.1007/s00248-018-1284-z

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Keywords

  • Soil bacteria
  • Community assembly
  • Microcosm
  • Regional processes
  • Seed addition