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Microbial Community Composition and Putative Biogeochemical Functions in the Sediment and Water of Tropical Granite Quarry Lakes

Microbial Ecology volume 77, pages 1–11 (2019)Cite this article

Abstract

Re-naturalized quarry lakes are important ecosystems, which support complex communities of flora and fauna. Microorganisms associated with sediment and water form the lowest trophic level in these ecosystems and drive biogeochemical cycles. A direct comparison of microbial taxa in water and sediment microbial communities is lacking, which limits our understanding of the dominant functions that are carried out by the water and sediment microbial communities in quarry lakes. In this study, using the 16S rDNA amplicon sequencing approach, we compared microbial communities in the water and sediment in two re-naturalized quarry lakes in Singapore and elucidated putative functions of the sediment and water microbial communities in driving major biogeochemical processes. The richness and diversity of microbial communities in sediments of the quarry lakes were higher than those in the water. The composition of the microbial communities in the sediments from the two quarries was highly similar to one another, while those in the water differed greatly. Although the microbial communities of the sediment and water samples shared some common members, a large number of microbial taxa (at the phylum and genus levels) were prevalent either in sediment or water alone. Our results provide valuable insights into the prevalent biogeochemical processes carried out by water and sediment microbial communities in tropical granite quarry lakes, highlighting distinct microbial processes in water and sediment that contribute to the natural purification of the resident water.

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Acknowledgements

Sequencing was carried out with the help of Dr. Daniela Moses and Professor Stephan Schuster using the sequencing facilities at the Singapore Centre for Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore. We thank the Singapore National Parks Board (NParks), in particular, Ms. Samantha Lai and Ms. Joanna Yeo, for their assistance in sample collection. This material is based on research/work supported by the Singapore Ministry of National Development and National Research Foundation under L2 NIC Award No. L2NICCFP1-2013-3.

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  1. Amit Kumar and Daphne H. P. Ng contributed equally to this work.

Authors and Affiliations

  1. School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Ave, N1-01C-69, Singapore, 639798, Singapore

    Amit Kumar, Daphne H. P. Ng & Bin Cao

  2. Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore

    Amit Kumar, Daphne H. P. Ng & Bin Cao

  3. College of Resources and Environment, Huazhong Agricultural University, No. 1 Shizishan Street, Wuhan, 430070, China

    Yichao Wu

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  1. Amit Kumar
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Correspondence to Bin Cao.

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Kumar, A., Ng, D.H.P., Wu, Y. et al. Microbial Community Composition and Putative Biogeochemical Functions in the Sediment and Water of Tropical Granite Quarry Lakes. Microb Ecol 77, 1–11 (2019). https://doi.org/10.1007/s00248-018-1204-2

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Keywords

  • Quarry lake
  • Sediment
  • 16S rDNA amplicon sequencing
  • Microbial community