World’s Largest Mass Bathing Event Influences the Bacterial Communities of Godavari, a Holy River of India
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Kumbh Mela is one of the largest religious mass gathering events (MGE) involving bathing in rivers. The exponential rise in the number of devotees, from around 0.4 million in 1903 to 120 million in 2013, bathing in small specified sites can have a dramatic impact on the river ecosystem. Here, we present the spatiotemporal profiling of bacterial communities in Godavari River, Nashik, India, comprising five sites during the Kumbh Mela, held in 2015. Assessment of environmental parameters indicated deterioration of water quality. Targeted amplicon sequencing demonstrates approximately 37.5% loss in microbial diversity because of anthropogenic activity during MGE. A significant decrease in phyla viz. Actinobacteria, Chloroflexi, Proteobacteria, and Bacteroidetes was observed, while we noted substantial increase in prevalence of the phylum Firmicutes (94.6%) during MGE. qPCR estimations suggested nearly 130-fold increase in bacterial load during the event. Bayesian mixing model accounted the source of enormous incorporation of bacterial load of human origin. Further, metagenomic imputations depicted increase in virulence and antibiotic resistance genes during the MGE. These observations suggest the striking impact of the mass bathing on river ecosystem. The subsequent increase in infectious diseases and drug-resistant microbes pose a critical public health concern.
Keywords16S rRNA gene Kumbh Mela Antibiotic resistance Disease outbreak Public health Godavari River
We would like to acknowledge director NCCS for providing infrastructure. We are grateful to Dr. Vilas Sinkar for providing valuable comments and critical review of the manuscript. Mr. Vishal Nawale is highly acknowledged for his help in sampling.
K.J., J.B., Y.S. and A.S. were involved in the study design; J.B. was involved in the sample collection; K.J., J.B., Y.S. M.S. and A.S. were involved in the experimental work; K.J., D.D., Y.S., and A.S. were involved in the bioinformatics and statistical analysis; K.J., D.D., Y.S., V. R. and A.S interpretation of the data; K.J., D.D., Y.S., V. R. and A.S. were involved in the drafting of the manuscript; K.J., D.D., Y.S., V. R. and A.S were involved in the critical revision of the manuscript.
This work was supported by the “Department of Biotechnology (DBT), Government of India” (by Grant no. BT/PR/0054/NDB/52/94/2007), under the project “Establishment of Microbial Culture Collection.”
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no competing interests.
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