Microbial Ecology

, Volume 76, Issue 1, pp 182–191 | Cite as

Microbial Reduction of Fe(III) and SO4 2− and Associated Microbial Communities in the Alluvial Aquifer Groundwater and Sediments

  • Ji-Hoon LeeEmail author
  • Bong-Joo Lee
Environmental Microbiology


Agricultural demands continuously increased use of groundwater, causing drawdown of water table and need of artificial recharge using adjacent stream waters. River water intrusion into groundwater can alter the geochemical and microbiological characteristics in the aquifer and subsurface. In an effort to investigate the subsurface biogeochemical activities before operation of artificial recharge at the test site, established at the bank of Nakdong River, Changwon, South Korea, organic carbon transported from river water to groundwater was mimicked and the effect on the indigenous microbial communities was investigated with the microcosm incubations of the groundwater and subsurface sediments. Laboratory incubations indicated microbial reduction of Fe(III) and sulfate. Next-generation Illumina MiSeq sequences of V4 region of 16S rRNA gene provided that the shifts of microbial taxa to Fe(III)-reducing and/or sulfate-reducing microorganisms such as Geobacter, Albidiferax, Desulfocapsa, Desulfuromonas, and Desulfovibrio were in good correlation with the sequential flourishment of microbial reduction of Fe(III) and sulfate as the incubations progressed. This suggests the potential role of dissolved organic carbons migrated with the river water into groundwater in the managed aquifer recharge system on the indigenous microbial community composition and following alterations of subsurface biogeochemistry and microbial metabolic activities.


Organic carbon Groundwater recharge Microbial community 


Funding Information

This work was supported by the Korea Institute of Geoscience and Mineral Resources (Basic Research Project No. 14-3211) funded by the Ministry of Science, ICT and Future Planning and also in part by the Cooperative Research Program for Agricultural Science and Technology Development (Project No. PJ012263), Rural Development Administration, Republic of Korea.

Supplementary material

248_2017_1119_MOESM1_ESM.docx (5 mb)
ESM 1 (DOCX 5155 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Bioenvironmental ChemistryChonbuk National UniversityJeonjuRepublic of Korea
  2. 2.Groundwater DepartmentKorea Institute of Geoscience and Mineral ResourcesDaejeonRepublic of Korea

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