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Distinctive Soil Archaeal Communities in Different Variants of Tropical Equatorial Forest

  • Soil Microbiology
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Abstract

Little is known of how soil archaeal community composition and diversity differ between local variants of tropical rainforests. We hypothesized that (1) as with plants, animals, fungi, and bacteria, the soil archaeal community would differ between different variants of tropical forest; (2) that spatially rarer forest variants would have a less diverse archaeal community than common ones; (3) that a history of forest disturbance would decrease archaeal alpha- and beta-diversity; and (4) that archaeal distributions within the forest would be governed more by deterministic than stochastic factors. We sampled soil across several different forest types within Brunei, Northwest Borneo. Soil DNA was extracted, and the 16S rRNA gene of archaea was sequenced using Illumina MiSeq. We found that (1) as hypothesized, there are distinct archaeal communities for each forest type, and community composition significantly correlates with soil parameters including pH, organic matter, and available phosphorous. (2) As hypothesized, the “rare” white sand forest variants kerangas and inland heath had lower archaeal diversity. A nestedness analysis showed that archaeal community in inland heath and kerangas was mainly a less diverse subset of that in dipterocarp forests. However, primary dipterocarp forest had the lowest beta-diversity among the other tropical forest types. (3) Also, as predicted, forest disturbance resulted in lower archaeal alpha-diversity—but increased beta-diversity in contrast with our predictions. (4) Contrary to our predictions, the BetaNTI of the various primary forest types indicated community assembly was mainly stochastic. The possible effects of these habitat and disturbance-related effects on N cycling should be investigated.

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Acknowledgments

This work was supported by a grant from the National Research Foundation (NRF) funded by the Korean Government, Ministry of Education, Science and Technology (MEST) (NRF-0409-20150076). We thank the Brunei Forestry Department and the Biodiversity Research and Innovation Centre for entry and export permits, respectively, and the Universiti Brunei Darussalam for permission to conduct research.

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  1. Dorsaf Kerfahi

    Present address: Biological Oceanography, Leibniz Institute for Baltic Sea Research Warnemünde (IOW), Seestrasse 15, 18119, Rostock, Germany

Authors and Affiliations

  1. Department of Biological Sciences, Seoul National University, Gwanak-Gu, Seoul, 151-747, Republic of Korea

    Dorsaf Kerfahi

  2. Korea Polar Research Institute, 26 Songdomirae-ro, Yeonsu-gu, Incheon, 21990, Republic of Korea

    Binu M. Tripathi

  3. Faculty of Science, Universiti Brunei Darussalam, Gadong, Brunei Darussalam

    J. W. Ferry Slik, Rahayu S. Sukri & Salwana Jaafar

  4. Division of Agrifood and Environment, Cranfield University, College Rd, Cranfield, MK43 0AL, UK

    Jonathan M. Adams

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  1. Dorsaf Kerfahi
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Correspondence to Jonathan M. Adams.

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Kerfahi, D., Tripathi, B.M., Slik, J.W.F. et al. Distinctive Soil Archaeal Communities in Different Variants of Tropical Equatorial Forest. Microb Ecol 76, 215–225 (2018). https://doi.org/10.1007/s00248-017-1118-4

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