Salinity Drives the Virioplankton Abundance but Not Production in Tropical Coastal Lagoons

  • Pedro C. Junger
  • André M. Amado
  • Rodolfo Paranhos
  • Anderson S. Cabral
  • Saulo M. S. Jacques
  • Vinicius F. Farjalla
Microbiology of Aquatic Systems

Abstract

Viruses are the most abundant components of microbial food webs and play important ecological and biogeochemical roles in aquatic ecosystems. Virioplankton is regulated by several environmental factors, such as salinity, turbidity, and humic substances. However, most of the studies aimed to investigate virioplankton regulation were conducted in temperate systems combining a limited range of environmental variables. In this study, virus abundance and production were determined and their relation to bacterial and limnological variables was assessed in 20 neighboring shallow tropical coastal lagoons that present wide environmental gradients of turbidity (2.32–571 NTU), water color (1.82–92.49 m−1), dissolved organic carbon (0.71–16.7 mM), salinity (0.13–332.1‰), and chlorophyll-a (0.28 to 134.5 μg L−1). Virus abundance varied from 0.37 × 108 to 117 × 108 virus-like-particle (VLP) mL−1, with the highest values observed in highly salty aquatic systems. Salinity and heterotrophic bacterial abundance were the main variables positively driving viral abundances in these lagoons. We suggest that, with increased salinity, there is a decrease in the protozoan control on bacterial populations and lower bacterial diversity (higher encounter rates with virus specific hosts), both factors positively affecting virus abundance. Virus production varied from 0.68 × 107 to 56.5 × 107 VLP mL−1 h−1 and was regulated by bacterial production and total phosphorus, but it was not directly affected by salinity. The uncoupling between virus abundance and virus production supports that the hypothesis that the lack of grazing pressure on viral and bacterial populations is an important mechanism causing virus abundance to escalate with increasing salt concentrations.

Keywords

Virus-bacteria dynamics Virus shunt Dilution technique Flow cytometry Carbon cycling Shallow lakes 

Notes

Acknowledgments

PCJ is grateful to Brazilian Council for Research, Development and Innovation (CNPq) for a Master scholarship. VFF is grateful to CNPq for research funds and a productivity grant (Project 306448/2011-4). Authors are thankful to Alice Campos and Thiago Benevides for laboratory and fieldwork assistance, Jean Remy Guimarães for assistance in bacterial production analyses, and Fernanda Ferreira and Anderson dos Santos Junior for support in flow cytometry analyses. The authors also thank Andrew MacDonald for language review and Hugo Sarmento for his critical reading of an earlier version of this manuscript.

Supplementary material

248_2017_1038_MOESM1_ESM.docx (22 kb)
ESM 1(DOCX 21 kb)

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Pedro C. Junger
    • 1
    • 2
  • André M. Amado
    • 3
    • 4
  • Rodolfo Paranhos
    • 5
  • Anderson S. Cabral
    • 5
  • Saulo M. S. Jacques
    • 1
    • 6
  • Vinicius F. Farjalla
    • 1
  1. 1.Lab. Limnologia, Departamento de Ecologia, Instituto de Biologia, Centro de Ciências da SaúdeUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Programa de Pós-Graduação em EcologiaUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  3. 3.Departamento de Biologia, Instituto de Ciências BiológicasUniversidade Federal de Juiz de ForaJuiz de ForaBrazil
  4. 4.Departamento de Oceanografia e Limnologia, Instituto de BiociênciasUniversidade Federal do Rio Grande do NorteNatalBrazil
  5. 5.Laboratório de Hidrobiologia, Instituto de BiologiaUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  6. 6.Programa de Pós-Graduação em Ecologia e EvoluçãoUniversidade Estadual do Rio de JaneiroRio de JaneiroBrazil

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