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Steady-State Oxygen Isotope Effects of N2O Production in Paracoccus denitrificans

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Abstract

Knowledge of isotopic discrimination, or fractionation, by denitrifying bacteria can benefit agricultural fertilizer management, wastewater treatment, and other applications. However, the complexity of N transformation pathways in the environment and the sensitivity of denitrification to environmental conditions warrant better isotopic distinction between denitrification and other processes, especially for oxygen isotopes. Here, we present a dataset of δ18O measurements in continuous culture of Paracoccus denitrificans. The authors hope that it will be useful in further studies of N2O in the environment.

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Authors and Affiliations

  1. University of Wisconsin–Madison, Madison, WI, USA

    Carol Barford

  2. Georgia Institute of Technology, Atlanta, GA, USA

    Joseph Montoya

  3. University of Massachusetts–Dartmouth, Darmouth, MA, USA

    Mark Altabet

  4. Harvard University, Cambridge, MA, USA

    Ralph Mitchell

Authors
  1. Carol Barford
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  2. Joseph Montoya
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  3. Mark Altabet
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  4. Ralph Mitchell
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Corresponding author

Correspondence to Carol Barford.

Electronic Supplementary Material

Online Resource 1

This spreadsheet file gives the measured δ18O of N2O from six chemostat runs. Data shown are the fraction of NO3− consumed at steady state, and the δ18O of N2O relative to two common standards (SMOW and atmospheric O2). The number of days elapsed between sample collection and cryogenic distillation are also shown. (XLSX 32 kb)

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Barford, C., Montoya, J., Altabet, M. et al. Steady-State Oxygen Isotope Effects of N2O Production in Paracoccus denitrificans . Microb Ecol 74, 507–509 (2017). https://doi.org/10.1007/s00248-017-0965-3

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  • DOI: https://doi.org/10.1007/s00248-017-0965-3

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