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Fungi as Architects of the Rimstone Dams in Huanglong, NSD, Sichuan, China

  • Microbiology of Aquatic Systems
  • Published:
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Abstract

The Huanglong park area of the Sichuan Province of China is a unique scenic area of the world. It is known for its thousands of aquamarine-colored pools that are formed behind naturally formed rimstone dams of travertine (calcite) along a cold water stream. The travertine, based on its crystalline structural analysis, is of biological origin. This makes sense since the temperature of the waters of Huanglong varies from 5 to 7 °C and thus geochemical crystallization does not occur as it does in other locations around the world possessing thermal pools whose structures are primarily formed through cooling processes. Fungi and bacteria were discovered associated with both leaves associated with the calcite dams as well as in the older parts of well-established dams. Several species of Phytium, a phycomycete and an endophyte, accounted for over 45 % of all of the fungi successfully isolated from the well-established dam samples and at least 85 % in the floating leaf samples. Saprolegnia spp. (Phycomycetes) along with Phoma spp. (Ascomycetes) were noted along with Mortierella sp. as other dam-associated fungi. The fungal hyphae observed on dead leaf material as well as in the calcite dams directly served as nucleation points for the formation of crystalline CaCO3. Eventually, these crystals grow large enough to fuse to make calcite plates which form the main structural feature of all of the travertine dams in this area. Interestingly, each of the individual crystals associated with the dams has an associated hole in its core where a fungal hypha used to reside as observed by scanning electron microscopy. While diatoms were present in the analysis, they too seem to contribute to the structure of the dams but in a minor way. The only bacteria isolated from the older dam of this aquatic environment were Pseudomonas spp. and their role in dam formation is uncertain. Huanglong is a unique and beautiful place, and the water features present in this area can definitely be attributed to those fungal architects that encourage calcite crystal formation.

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Acknowledgments

The authors acknowledge the financial support of the Natural Science Foundation of Chongqing (cstc2015jcyjys80001) to Jie Xie.

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Authors and Affiliations

  1. State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing, 400715, People’s Republic of China

    Jie Xie, Wei-Fang Xu, Jie Chen & Hui-Shuang Ren

  2. Department of Plant Sciences, Montana State University, Bozeman, MT, 59717, USA

    Gary Strobel

  3. Huanglong National Scenic Resort Administrative Bureau, Songpan, Sichuan, 623300, People’s Republic of China

    De-Jun An

  4. Department of Plant and Wildlife Sciences, Brigham Young University, Provo, UT, 84602, USA

    Brad Geary

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  1. Jie Xie
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  2. Gary Strobel
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  3. Wei-Fang Xu
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  4. Jie Chen
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  5. Hui-Shuang Ren
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  6. De-Jun An
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  7. Brad Geary
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Correspondence to Gary Strobel.

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Xie, J., Strobel, G., Xu, WF. et al. Fungi as Architects of the Rimstone Dams in Huanglong, NSD, Sichuan, China. Microb Ecol 73, 29–38 (2017). https://doi.org/10.1007/s00248-016-0841-6

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  • DOI: https://doi.org/10.1007/s00248-016-0841-6

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