Microbial Ecology

, Volume 72, Issue 4, pp 943–954

Effect of Antibiotic Treatment on the Gastrointestinal Microbiome of Free-Ranging Western Lowland Gorillas (Gorilla g. gorilla)

  • Klára Vlčková
  • Andres Gomez
  • Klára J. Petrželková
  • Christopher A. Whittier
  • Angelique F. Todd
  • Carl J. Yeoman
  • Karen E. Nelson
  • Brenda A. Wilson
  • Rebecca M. Stumpf
  • David Modrý
  • Bryan A. White
  • Steven R. Leigh
Host Microbe Interactions

Abstract

The mammalian gastrointestinal (GI) microbiome, which plays indispensable roles in host nutrition and health, is affected by numerous intrinsic and extrinsic factors. Among them, antibiotic (ATB) treatment is reported to have a significant effect on GI microbiome composition in humans and other animals. However, the impact of ATBs on the GI microbiome of free-ranging or even captive great apes remains poorly characterized. Here, we investigated the effect of cephalosporin treatment (delivered by intramuscular dart injection during a serious respiratory outbreak) on the GI microbiome of a wild habituated group of western lowland gorillas (Gorilla gorilla gorilla) in the Dzanga Sangha Protected Areas, Central African Republic. We examined 36 fecal samples from eight individuals, including samples before and after ATB treatment, and characterized the GI microbiome composition using Illumina-MiSeq sequencing of the bacterial 16S rRNA gene. The GI microbial profiles of samples from the same individuals before and after ATB administration indicate that the ATB treatment impacts GI microbiome stability and the relative abundance of particular bacterial taxa within the colonic ecosystem of wild gorillas. We observed a statistically significant increase in Firmicutes and a decrease in Bacteroidetes levels after ATB treatment. We found disruption of the fibrolytic community linked with a decrease of Ruminoccocus levels as a result of ATB treatment. Nevertheless, the nature of the changes observed after ATB treatment differs among gorillas and thus is dependent on the individual host. This study has important implications for ecology, management, and conservation of wild primates.

Keywords

Gorilla Antibiotics Medical treatment Gastrointestinal microbiome Illumina MiSeq Bacteria 

Supplementary material

248_2016_745_MOESM1_ESM.docx (16 kb)
ESM 1(DOCX 16.3 kb)
248_2016_745_MOESM2_ESM.docx (35 kb)
ESM 2(DOCX 29.0 kb)
248_2016_745_MOESM3_ESM.docx (128 kb)
ESM 3(DOCX 13 kb)
248_2016_745_MOESM4_ESM.docx (46 kb)
ESM 4(DOCX 13 kb)
248_2016_745_MOESM5_ESM.docx (14 kb)
ESM 5(DOCX 13 kb)
248_2016_745_MOESM6_ESM.docx (40 kb)
ESM 6(DOCX 13 kb)
248_2016_745_MOESM7_ESM.docx (32 kb)
ESM 7(DOCX 235 kb)
248_2016_745_MOESM8_ESM.docx (11 kb)
ESM 8(DOCX 91 kb)
248_2016_745_MOESM9_ESM.docx (11 kb)
ESM 9(DOCX 19 kb)
248_2016_745_MOESM10_ESM.docx (11 kb)
ESM 10(DOCX 129 kb)
248_2016_745_MOESM11_ESM.docx (13 kb)
ESM 11(DOCX 57 kb)
248_2016_745_MOESM12_ESM.docx (29 kb)
ESM 12(DOCX 318 kb)

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Klára Vlčková
    • 1
  • Andres Gomez
    • 2
    • 3
  • Klára J. Petrželková
    • 4
    • 5
    • 6
  • Christopher A. Whittier
    • 7
    • 8
  • Angelique F. Todd
    • 9
  • Carl J. Yeoman
    • 10
  • Karen E. Nelson
    • 11
    • 16
  • Brenda A. Wilson
    • 3
    • 12
  • Rebecca M. Stumpf
    • 3
    • 13
  • David Modrý
    • 1
    • 6
    • 14
  • Bryan A. White
    • 3
  • Steven R. Leigh
    • 3
    • 15
  1. 1.Department of Pathology and Parasitology, Faculty of Veterinary MedicineUniversity of Veterinary and Pharmaceutical Sciences BrnoBrnoCzech Republic
  2. 2.J. Craig Venter InstituteLa JollaUSA
  3. 3.Carl R. Woese Institute for Genomic BiologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  4. 4.Institute of Vertebrate BiologyAcademy of Sciences of the Czech RepublicBrnoCzech Republic
  5. 5.Liberec ZooLiberecCzech Republic
  6. 6.Institute of ParasitologyBiology Centre of the Academy of Sciences of the Czech RepublicČeské BudějoviceCzech Republic
  7. 7.Cummings School of Veterinary Medicine at Tufts UniversityNorth GraftonUSA
  8. 8.Department of Wildlife Health SciencesSmithsonian Conservation Biology InstituteWashingtonUSA
  9. 9.WWF, Dzanga Sangha Protected AreasBanguiCentral African Republic
  10. 10.Department of Animal and Range SciencesMontana State UniversityBozemanUSA
  11. 11.J. Craig Venter InstituteRockvilleUSA
  12. 12.Department of MicrobiologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  13. 13.Department of AnthropologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  14. 14.CEITEC VFUUniversity of Veterinary and Pharmaceutical Sciences BrnoBrnoCzech Republic
  15. 15.Department of AnthropologyUniversity of Colorado at BoulderBoulderUSA
  16. 16.J. Craig Venter InstituteLa JollaUSA

Personalised recommendations