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Patterns of Multi-Antibiotic-Resistant Escherichia Coli from Streams with No History of Antimicrobial Inputs

Abstract

A growing body of evidence suggests that contaminated environments may harbor a greater proportion of antibiotic-resistant microorganisms than unpolluted reference sites. Here, we report the screening of 427 Escherichia coli strains isolated from 11 locations on nine streams draining the US Department of Energy’s Savannah River Site against a panel of five antibiotics. Streams were chosen to capture a wide range of watersheds from minimally disturbed to highly impacted. Overall, higher levels of resistance were found in waterborne E. coli that also generally exhibited low spatial variability. However, 3 of 11 locations also demonstrated elevated resistance levels in sediments. Two of these occurred in highly disturbed tributaries with no obvious sources of antimicrobials. To further investigate these patterns, we screened a subset of isolates obtained from three streams against 23 antibiotics or antibiotic combinations. A large proportion of these isolates (>40 %) demonstrated resistance to 10 or more antimicrobials, suggesting that environmental multi-antibiotic resistance may be prevalent in this bacterial commensal. Only 4 of 87 viable isolates were tested susceptible to all 23 antibiotics and combinations. Among these multi-antibiotic-resistant isolates, several demonstrated resistance to all structural classes of antimicrobial agents tested, including frontline antibiotics such as gatifloxacin and ciprofloxacin.

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Acknowledgments

We thank Paul Stankus and Angela Lindell for technical support and advice. Financial support was provided from the US Department of Energy Financial Assistance Award no. DE-FC09-96SR18546 to the University of Georgia Research Foundation.

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Correspondence to J. V. McArthur.

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figure6

S1. Proportions of isolates from U4, U8, and U10 resistant to various antibiotic and antibiotic combinations excluding the cephlasporins. (GIF 239 kb)

figure7

S2. Proportions of isolates from U4, U8, and U10 resistant to various cephalasporins. (GIF 163 kb)

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High resolution image (TIFF 83 kb)

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McArthur, J.V., Fletcher, D.E., Tuckfield, R.C. et al. Patterns of Multi-Antibiotic-Resistant Escherichia Coli from Streams with No History of Antimicrobial Inputs. Microb Ecol 72, 840–850 (2016). https://doi.org/10.1007/s00248-015-0678-4

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Keywords

  • E. coli
  • Antibiotic resistance
  • Industrial activity