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Microbial Ecology

, Volume 72, Issue 4, pp 840–850 | Cite as

Patterns of Multi-Antibiotic-Resistant Escherichia Coli from Streams with No History of Antimicrobial Inputs

  • J. V. McArthurEmail author
  • D. E. Fletcher
  • R. Cary Tuckfield
  • C. Baker-Austin
Environmental Microbiology

Abstract

A growing body of evidence suggests that contaminated environments may harbor a greater proportion of antibiotic-resistant microorganisms than unpolluted reference sites. Here, we report the screening of 427 Escherichia coli strains isolated from 11 locations on nine streams draining the US Department of Energy’s Savannah River Site against a panel of five antibiotics. Streams were chosen to capture a wide range of watersheds from minimally disturbed to highly impacted. Overall, higher levels of resistance were found in waterborne E. coli that also generally exhibited low spatial variability. However, 3 of 11 locations also demonstrated elevated resistance levels in sediments. Two of these occurred in highly disturbed tributaries with no obvious sources of antimicrobials. To further investigate these patterns, we screened a subset of isolates obtained from three streams against 23 antibiotics or antibiotic combinations. A large proportion of these isolates (>40 %) demonstrated resistance to 10 or more antimicrobials, suggesting that environmental multi-antibiotic resistance may be prevalent in this bacterial commensal. Only 4 of 87 viable isolates were tested susceptible to all 23 antibiotics and combinations. Among these multi-antibiotic-resistant isolates, several demonstrated resistance to all structural classes of antimicrobial agents tested, including frontline antibiotics such as gatifloxacin and ciprofloxacin.

Keywords

E. coli Antibiotic resistance Industrial activity 

Notes

Acknowledgments

We thank Paul Stankus and Angela Lindell for technical support and advice. Financial support was provided from the US Department of Energy Financial Assistance Award no. DE-FC09-96SR18546 to the University of Georgia Research Foundation.

Supplementary material

248_2015_678_Fig6_ESM.gif (239 kb)

S1. Proportions of isolates from U4, U8, and U10 resistant to various antibiotic and antibiotic combinations excluding the cephlasporins. (GIF 239 kb)

248_2015_678_MOESM1_ESM.tif (173 kb)
High resolution image (TIFF 173 kb)
248_2015_678_Fig7_ESM.gif (163 kb)

S2. Proportions of isolates from U4, U8, and U10 resistant to various cephalasporins. (GIF 163 kb)

248_2015_678_MOESM2_ESM.tif (83 kb)
High resolution image (TIFF 83 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Savannah River Ecology LaboratoryUniversity of GeorgiaAikenUSA
  2. 2.Ecostatys, LLCAikenUSA
  3. 3.Centre for EnvironmentFisheries and Aquaculture ScienceDorsetUK

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