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Microbial Ecology

, Volume 69, Issue 3, pp 586–596 | Cite as

Community Composition of Known and Uncultured Archaeal Lineages in Anaerobic or Anoxic Wastewater Treatment Sludge

  • Kyohei Kuroda
  • Masashi Hatamoto
  • Nozomi Nakahara
  • Kenichi Abe
  • Masanobu Takahashi
  • Nobuo Araki
  • Takashi YamaguchiEmail author
Environmental Microbiology

Abstract

Microbial systems are widely used to treat different types of wastewater from domestic, agricultural, and industrial sources. Community composition is an important factor in determining the successful performance of microbial treatment systems; however, a variety of uncultured and unknown lineages exist in sludge that requires identification and characterization. The present study examined the archaeal community composition in methanogenic, denitrifying, and nitrogen-/phosphate-removing wastewater treatment sludge by Archaea-specific 16S rRNA gene sequencing analysis using Illumina sequencing technology. Phylotypes belonging to Euryarchaeota, including methanogens, were most abundant in all samples except for nitrogen-/phosphate-removing wastewater treatment sludge. High levels of Deep Sea Hydrothermal Vent Group 6 (DHVEG-6), WSA2, Terrestrial Miscellaneous Euryarchaeotal Group, and Miscellaneous Crenarchaeotic Group were also detected. Interestingly, DHVEG-6 was dominant in nitrogen-/phosphate-removing wastewater treatment sludge, indicating that unclear lineages of Archaea still exist in the anaerobic wastewater treatment sludges. These results reveal a previously unknown diversity of Archaea in sludge that can potentially be exploited for the development of more efficient wastewater treatment strategies.

Keywords

Archaeal community composition Uncultured lineages 16S rRNA gene sequencing Diversity analysis Anaerobic wastewater treatment sludge 

Notes

Acknowledgments

This research was supported in part by research grants from the Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT), the Japan Society for the Promotion of Science (JSPS), and the Science and Technology Research Partnership for Sustainable Development (SATREPS). The authors thank Aqua and Soil Environmental laboratory members of Aida A.A. and Maharajan N. for the critical reading of the manuscript; Furukawa A., Ohtsuki K., Chosei T., Okabe Y., Sakamoto K. and Watari T. for the assistance with the reactor operation; and Sato T. and Yamamoto M. for supporting the experiment and stimulating discussions.

Supplementary material

248_2014_525_MOESM1_ESM.docx (1.5 mb)
ESM 1 (DOCX 1512 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Kyohei Kuroda
    • 1
  • Masashi Hatamoto
    • 1
  • Nozomi Nakahara
    • 1
  • Kenichi Abe
    • 1
    • 2
  • Masanobu Takahashi
    • 2
  • Nobuo Araki
    • 3
  • Takashi Yamaguchi
    • 1
    Email author
  1. 1.Department of Environmental Systems EngineeringNagaoka University of TechnologyNagaokaJapan
  2. 2.Department of Civil and Environmental EngineeringTohoku UniversitySendaiJapan
  3. 3.Department of Civil EngineeringNagaoka National College of TechnologyNagaokaJapan

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