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Pairwise Transcriptomic Analysis of the Interactions Between the Ectomycorrhizal Fungus Laccaria bicolor S238N and Three Beneficial, Neutral and Antagonistic Soil Bacteria

Microbial Ecology volume 69, pages 146–159 (2015)Cite this article

Abstract

Ectomycorrhizal fungi are surrounded by bacterial communities with which they interact physically and metabolically during their life cycle. These bacteria can have positive or negative effects on the formation and the functioning of ectomycorrhizae. However, relatively little is known about the mechanisms by which ectomycorrhizal fungi and associated bacteria interact. To understand how ectomycorrhizal fungi perceive their biotic environment and the mechanisms supporting interactions between ectomycorrhizal fungi and soil bacteria, we analysed the pairwise transcriptomic responses of the ectomycorrhizal fungus Laccaria bicolor (Basidiomycota: Agaricales) when confronted with beneficial, neutral or detrimental soil bacteria. Comparative analyses of the three transcriptomes indicated that the fungus reacted differently to each bacterial strain. Similarly, each bacterial strain produced a specific and distinct response to the presence of the fungus. Despite these differences in responses observed at the gene level, we found common classes of genes linked to cell–cell interaction, stress response and metabolic processes to be involved in the interaction of the four microorganisms.

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Acknowledgments

A. Deveau was supported by a scholarship from INRA, Lorraine Region and the Foreign Office exchange program Van Gogh. This work was supported by the French National Research Agency through the Laboratory of Excellence ARBRE (ANR-11-LABX-0002-01). We would like to thank A. Kohler for her help in submitting data to the GEO database and Aimee Orsini for proofreading the manuscript.

Author information

Author notes

  1. Matthieu Barret

    Present address: INRA – IRHS, 42 rue Georges Morel, 49071, Beaucouze, France

  2. Abdala G. Diedhiou

    Present address: Laboratoire Commun de Microbiologie, IRD/ISRA/UCAD Centre de Recherche de Bel-Air, BP1386, 18524, Dakar, Senegal

Affiliations

  1. Interactions Arbres – Microorganismes, INRA UMR1136, 54280, Champenoux, France

    Aurélie Deveau, Abdala G. Diedhiou, Francis Martin & Pascale Frey-Klett

  2. Interactions Arbres – Microorganismes, Université de Lorraine UMR1136, 54500, Vandoeuvre-lès-Nancy, France

    Aurélie Deveau, Abdala G. Diedhiou, Francis Martin & Pascale Frey-Klett

  3. INRA UMR1349 IGEPP, 35653, Le Rheu, France

    Matthieu Barret & Alain Sarniguet

  4. Department of Plant Pathology, University of California, Davis, CA, 95616, USA

    Johan Leveau

  5. Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, The Netherlands

    Wietse de Boer

  6. Interactions Arbres Micro-organismes, INRA Lorraine Université, UMR 1136, 54280, Champenoux, France

    Aurélie Deveau

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  1. Aurélie Deveau
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Correspondence to Aurélie Deveau.

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Deveau, A., Barret, M., Diedhiou, A.G. et al. Pairwise Transcriptomic Analysis of the Interactions Between the Ectomycorrhizal Fungus Laccaria bicolor S238N and Three Beneficial, Neutral and Antagonistic Soil Bacteria. Microb Ecol 69, 146–159 (2015). https://doi.org/10.1007/s00248-014-0445-y

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  • DOI: https://doi.org/10.1007/s00248-014-0445-y

Keywords

  • Hypothetical Protein
  • Ectomycorrhizal Fungus
  • Primary Metabolism
  • Transcriptomic Response
  • Saccharopine