Pairwise Transcriptomic Analysis of the Interactions Between the Ectomycorrhizal Fungus Laccaria bicolor S238N and Three Beneficial, Neutral and Antagonistic Soil Bacteria
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Ectomycorrhizal fungi are surrounded by bacterial communities with which they interact physically and metabolically during their life cycle. These bacteria can have positive or negative effects on the formation and the functioning of ectomycorrhizae. However, relatively little is known about the mechanisms by which ectomycorrhizal fungi and associated bacteria interact. To understand how ectomycorrhizal fungi perceive their biotic environment and the mechanisms supporting interactions between ectomycorrhizal fungi and soil bacteria, we analysed the pairwise transcriptomic responses of the ectomycorrhizal fungus Laccaria bicolor (Basidiomycota: Agaricales) when confronted with beneficial, neutral or detrimental soil bacteria. Comparative analyses of the three transcriptomes indicated that the fungus reacted differently to each bacterial strain. Similarly, each bacterial strain produced a specific and distinct response to the presence of the fungus. Despite these differences in responses observed at the gene level, we found common classes of genes linked to cell–cell interaction, stress response and metabolic processes to be involved in the interaction of the four microorganisms.
KeywordsHypothetical Protein Ectomycorrhizal Fungus Primary Metabolism Transcriptomic Response Saccharopine
A. Deveau was supported by a scholarship from INRA, Lorraine Region and the Foreign Office exchange program Van Gogh. This work was supported by the French National Research Agency through the Laboratory of Excellence ARBRE (ANR-11-LABX-0002-01). We would like to thank A. Kohler for her help in submitting data to the GEO database and Aimee Orsini for proofreading the manuscript.
- 5.Smith S, Read D (2008) Mycorrhizal symbiosis, 3rd edn. Mycorrhizal symbiosis 800Google Scholar
- 9.Riedlinger J, Schrey SD, Tarkka MT et al (2006) Auxofuran, a novel metabolite that stimulates the growth of fly agaric, is produced by the mycorrhiza helper bacterium Streptomyces strain AcH 505. Appl Environ Microbiol 72:3550–3557. doi: 10.1128/AEM.72.5.3550-3557.2006 PubMedCentralPubMedCrossRefGoogle Scholar
- 16.Sambrook J, Fritsch E, Maniatis T (1989) Molecular cloning: a laboratory manual. 1626Google Scholar
- 20.Barret M, Frey-Klett P, Guillerm-Erckelboudt AY et al (2009) Effect of wheat roots infected with the pathogenic fungus Gaeumannomyces graminis var. tritici on gene expression of the biocontrol bacterium Pseudomonas fluorescens Pf29Arp. Mol Plant Microbe Interact 22:1611–1623PubMedCrossRefGoogle Scholar
- 31.Wohlschlager T, Butschi A, Grassi P et al (2014) Methylated glycans as conserved targets of animal and fungal innate defense. Proc Natl Acad Sci USA. doi: 10.1073/pnas.1401176111
- 34.Daval S, Lebreton L, Gazengel K et al (2011) The biocontrol bacterium Pseudomonas fluorescens Pf29Arp strain affects the pathogenesis-related gene expression of the take-all fungus Gaeumannomyces graminis var. tritici on wheat roots. Mol Plant Pathol 12:839–854PubMedCentralPubMedCrossRefGoogle Scholar
- 35.Cumming J, Swiger T, Kurnik B, Panaccione D (2001) Organic acid exudation by Laccaria bicolor and Pisolithus tinctorius exposed to aluminum in vitro. Can J For Res 31:703–710Google Scholar