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Changes in the Soil Bacterial Communities in a Cedar Plantation Invaded by Moso Bamboo

  • Soil Microbiology
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Abstract

Moso bamboo is fast-growing and negatively allelopathic to neighboring plants. However, there is little information on the effects of its establishment and expansion to adjacent forest soil communities. To better understand the impacts of bamboo invasion on soil communities, the phylogenetic structure and diversity of the soil bacterial communities in moso bamboo forest, adjacent Japanese cedar plantation, and bamboo-invaded transition zone were examined using a combination of 16S rRNA gene clone libraries and bar-coded pyrosequencing techniques. Based on the number of operational taxonomic units (OTUs), Shannon diversity index, Chao1 estimator, and rarefaction analysis of both techniques, the bamboo soil bacterial community was the most diverse, followed by the transition zone, with the cedar plantation possessing the lowest diversity. The results from both techniques revealed that the Acidobacteria and Proteobacteria predominated in the three communities, though the relative abundance was different. The 250 most abundant OTUs represented about 70 % of the total sequences found by pyrosequencing. Most of these OTUs were found in all three soil communities, demonstrating the overall similarity among the bacterial communities. Nonmetric multidimensional scaling analysis showed further that the bamboo and transition soil communities were more similar with each other than the cedar soils. These results suggest that bamboo invasion to the adjacent cedar plantation gradually increased the bacterial diversity and changed the soil community. In addition, while the 10 most abundant OTUs were distributed worldwide, related sequences were not abundant in soils from outside the forest studied here. This result may be an indication of the uniqueness of this region.

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Acknowledgments

This work was supported by the Taiwan National Science Council, Taiwan (NSC 101-2621-B-001-002-MY3).

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Authors and Affiliations

  1. Biodiversity Research Center, Academia Sinica, Nankang, Taipei, 11529, Taiwan

    Yu-Te Lin, Sen-Lin Tang & Chih-Yu Chiu

  2. The Experimental Forest, College of Bio-Resource and Agriculture, National Taiwan University, Nantou, 557-43, Taiwan

    Chuang-Wen Pai

  3. Department of Microbiology, University of Georgia, Athens, GA, 30602-2605, USA

    William B. Whitman

  4. Odum School of Ecology, University of Georgia, Athens, GA, 30602-2602, USA

    David C. Coleman

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  1. Yu-Te Lin
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  2. Sen-Lin Tang
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  3. Chuang-Wen Pai
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  6. Chih-Yu Chiu
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Correspondence to William B. Whitman or Chih-Yu Chiu.

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Supplementary Fig. 1

(a) Aerial view of cedar (dark green) and bamboo forests (light green). (b) Bamboo-invaded transition zone. (c) Bamboo forest. Bamboo stems were occasionally cut by farmers and lot of stumps are in the forest. (d) Cedar forest. This forest is largely undisturbed and has lush understory plants. (e) Cross view from cedar (left) to bamboo (right) forest (DOC 771 kb)

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Lin, YT., Tang, SL., Pai, CW. et al. Changes in the Soil Bacterial Communities in a Cedar Plantation Invaded by Moso Bamboo. Microb Ecol 67, 421–429 (2014). https://doi.org/10.1007/s00248-013-0291-3

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