Can't Take the Heat: High Temperature Depletes Bacterial Endosymbionts of Ants
Members of the ant tribe Camponotini have coevolved with Blochmannia, an obligate intracellular bacterial mutualist. This endosymbiont lives within host bacteriocyte cells that line the ant midgut, undergoes maternal transmission from host queens to offspring, and contributes to host nutrition via nitrogen recycling and nutrient biosynthesis. While elevated temperature has been shown to disrupt obligate bacterial mutualists of some insects, its impact on the ant-Blochmannia partnership is less clear. Here, we test the effect of heat on the density of Blochmannia in two related Camponotus species in the lab. Transcriptionally active Blochmannia were quantified using RT-qPCR as the ratio of Blochmannia 16S rRNA to ant host elongation factor 1-α transcripts. Our results showed that 4 weeks of heat treatment depleted active Blochmannia by >99 % in minor workers and unmated queens. However, complete elimination of Blochmannia transcripts rarely occurred, even after 16 weeks of heat treatment. Possible mechanisms of observed thermal sensitivity may include extreme AT-richness and related features of Blochmannia genomes, as well as host stress responses. Broadly, the observed depletion of an essential microbial mutualist in heat-treated ants is analogous to the loss of zooanthellae during coral bleaching. While the ecological relevance of Blochmannia's thermal sensitivity is uncertain, our results argue that symbiont dynamics should be part of models predicting how ants and other animals will respond and adapt to a warming climate.
KeywordsMinor Worker rRNA Transcript Electronic Supplementary Material Supplementary Absolute Copy Number Female Alate
We thank Adam B. Lazarus for performing the MBL temperature experiments. We are grateful to Tatiana Fofanova and Bryan P. Brown for their help with NC ant collection and rearing. We thank Diana E. Wheeler for helpful discussion of temperature effects on endosymbionts. We appreciate the comments of four anonymous reviewers. Funding was provided by grants from the NSF (MCB-1103113) and NIH (R01GM062626) to JJW.
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