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Dynamics of Bacterial Communities in Cockles (Cerastoderma edule) with Respect to Trematode Parasite (Bucephalus minimus) Infestation

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Abstract

The bacterial communities associated with the cockle (Cerastoderma edule) were investigated at the individual level through a 10-month monitoring programme. Temporal changes and those changes associated with a common parasite of the cockle, Bucephalus minimus, were investigated by monthly sampling of individuals, selected based on their shell length (cohort monitoring). Cockle bacterial community abundance (CBCA) and diversity (CBCD) were estimated by epifluorescence microscopy counts and automated ribosomal intergenic spacer analysis, respectively. CBCA showed a temporal pattern peaking at 30 × 106 cells per gram of cockle flesh and intervalval liquid in October and a significant 1.8-fold increase linked with B. minimus occurrence. CBCD was characterized by 112 ± 26 intergenic transcribed spacer (ITS) per individual and showed a relative homology between individuals (52 ± 6%, Jaccard similarity) in spite of more than 30% of rare ITS. Consistent with an undisturbed evolution of the condition index of the studied cohort individuals as an estimate of their physiological state, neither temporal nor parasite-induced change in CBCA has been related to marked changes in CBCD.

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Acknowledgements

This study is part of the project “Multistress” (Coordinator: Dr. X. de Montaudouin) funded by Agence Nationale pour la Recherche, France. G. Meisterhans is funded by a doctoral fellowship of the Region Aquitaine. The authors thank SEPANSO for authorizing sampling in the National Reserve of Banc d’Arguin and Dr. S.C. Culloty for editing the English writing of the manuscript.

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Authors and Affiliations

  1. Université de Bordeaux UMR 5805 EPOC, station marine d’Arcachon, 2 rue du Pr Jolyet, 33120, Arcachon, France

    Guillaume Meisterhans, Natalie Raymond, Solène Lebreton, Line Bourasseau, Xavier de Montaudouin, Frédéric Garabetian & Florence Jude-Lemeilleur

  2. Plateforme Génôme-transcriptôme de Pierroton UMR BIOGECO 1202 INRA—Université Bordeaux 2, Bordeaux, France

    Franck Salin

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  1. Guillaume Meisterhans
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Correspondence to Florence Jude-Lemeilleur.

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Meisterhans, G., Raymond, N., Lebreton, S. et al. Dynamics of Bacterial Communities in Cockles (Cerastoderma edule) with Respect to Trematode Parasite (Bucephalus minimus) Infestation. Microb Ecol 62, 620–631 (2011). https://doi.org/10.1007/s00248-011-9865-0

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