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Occurrence and Identification of Yeast Species in Fermented Liquid Feed for Piglets

  • Environmental Microbiology
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Abstract

The major objective of the present study was to investigate the occurrence and identity of yeast species in fermented liquid feed (FLF) used for feeding piglets. In total, 40 different Danish farms were included in the analysis. The preparation and composition of FLF was found to be very heterogeneous with high variations in both yeast counts and yeast species composition. The yeast population varied between 6.0 × 103 and 4.2 × 107 cfug−1 with an average yeast count of 8.7 × 106 ± 1.1 × 107 cfug−1. A total of 766 yeasts were isolated and identified by conventional and/or molecular typing techniques. The predominant yeast species in the FLF samples were found to be Candida milleri (58.4%), Kazachstania exigua (17.5%), Candida pararugosa (6.40%) and Kazachstania bulderi (5.09%). No clear separation between isolates of C. milleri and Candida humilis could be obtained based on sequencing of the D1/D2 region of the 26S rRNA gene. The combined use of ITS-RFLP analysis and phenotypic criteria did meanwhile suggest a closer relationship with C. milleri than C. humilis.

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Acknowledgement

This work is supported by The Danish Ministry of Food, Agriculture and Fisheries.

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Authors and Affiliations

  1. Department of Food Science, Food Microbiology, Faculty of Life Sciences, University of Copenhagen, Rolighedsvej 30, 1958, Frederiksberg C, Denmark

    Klaus Gori, Marina Kryger Bjørklund & Lene Jespersen

  2. Department of Animal Health and Bioscience, Faculty of Agricultural Sciences, Aarhus University, Blichers Allé, P.O. Box 50, 8830, Tjele, Denmark

    Nuria Canibe

  3. Pig Research Center, Danish Agriculture and Food Council, Axeltorv 3, 1609, Copenhagen, V, Denmark

    Anni Øyan Pedersen

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  1. Klaus Gori
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  2. Marina Kryger Bjørklund
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  4. Anni Øyan Pedersen
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  5. Lene Jespersen
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Correspondence to Lene Jespersen.

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Gori, K., Kryger Bjørklund, M., Canibe, N. et al. Occurrence and Identification of Yeast Species in Fermented Liquid Feed for Piglets. Microb Ecol 61, 146–153 (2011). https://doi.org/10.1007/s00248-010-9706-6

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  • DOI: https://doi.org/10.1007/s00248-010-9706-6

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