Microbial Ecology

, Volume 59, Issue 3, pp 487–498 | Cite as

Molecular Ecology Of Macrolide–Lincosamide–Streptogramin B Methylases in Waste Lagoons and Subsurface Waters Associated with Swine Production

  • Satoshi Koike
  • Rustam I. Aminov
  • A. C. Yannarell
  • Holly D. Gans
  • Ivan G. Krapac
  • Joanne C. Chee-Sanford
  • Roderick I. Mackie
Environmental Microbiology

Abstract

RNA methylase genes are common antibiotic resistance determinants for multiple drugs of the macrolide, lincosamide, and streptogramin B (MLSB) families. We used molecular methods to investigate the diversity, distribution, and abundance of MLSB methylases in waste lagoons and groundwater wells at two swine farms with a history of tylosin (a macrolide antibiotic structurally related to erythromycin) and tetracycline usage. Phylogenetic analysis guided primer design for quantification of MLSB resistance genes found in tylosin-producing Streptomyces (tlr(B), tlr(D)) and commensal/pathogenic bacteria (erm(A), erm(B), erm(C), erm(F), erm(G), erm(Q)). The near absence of tlr genes at these sites suggested a lack of native antibiotic-producing organisms. The gene combination erm(ABCF) was found in all lagoon samples analyzed. These four genes were also detected with high frequency in wells previously found to be contaminated by lagoon leakage. A weak correlation was found between the distribution of erm genes and previously reported patterns of tetracycline resistance determinants, suggesting that dissemination of these genes into the environment is not necessarily linked. Considerations of gene origins in history (i.e., phylogeny) and gene distributions in the landscape provide a useful “molecular ecology” framework for studying environmental spread of antibiotic resistance.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Satoshi Koike
    • 1
    • 6
  • Rustam I. Aminov
    • 2
  • A. C. Yannarell
    • 1
    • 5
  • Holly D. Gans
    • 1
  • Ivan G. Krapac
    • 3
  • Joanne C. Chee-Sanford
    • 4
  • Roderick I. Mackie
    • 1
    • 5
  1. 1.Department of Animal SciencesUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  2. 2.Rowett Institute of Nutrition and HealthUniversity of AberdeenAberdeenUK
  3. 3.Illinois State Geological SurveyChampaignUSA
  4. 4.USDA Agricultural Research ServiceUrbanaUSA
  5. 5.Institute for Genomic BiologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  6. 6.Research Faculty of AgricultureHokkaido UniversitySapporoJapan

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