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Distribution of Cren- and Euryarchaeota in Scots Pine Mycorrhizospheres and Boreal Forest Humus

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Abstract

Archaeal 16S rRNA gene sequences have been found in a variety of moderate-temperature habitats including soil and rhizospheres. In this study, the differences of archaeal communities associated with Scots pine (Pinus sylvestris L.) short roots, different types of mycorrhizospheric compartments, and uncolonized boreal forest humus were tested by direct DNA extraction, polymerase chain reaction–denaturing gradient gel electrophoresis (PCR–DGGE), and sequencing. The results indicated that mycorrhizal colonization of Scots pine roots substantially influence the archaeal community of pine rhizospheres. Colonization of short roots by most mycorrhizal fungi tested increased both archaeal frequency and diversity. Most of the archaeal sequences encountered in mycorrhizas belonged to the phylum Euryarchaeota, order of Halobacteriales. The difference in archaeal diversity between the mycorrhizospheric compartments and humus was profound. Most compartments with fungal components contained euryarchaeotal 16S rRNA gene sequences, whereas a high diversity of crenarchaeotal sequences and no euryarchaeotal sequences were found in forest humus outside mycorrhizospheres.

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Acknowledgements

The Maj and Tor Nessling Foundation, Svenska Kulturfonden, University of Helsinki and Academy of Finland are thanked for financial support. Dr. Paula Kristo is thanked for her expert help in sequencing and Dr. Jodie Painter for her constructive comments on the manuscript. We also wish to warmly thank Professor Jari Valkonen for providing us with excellent working environment both in atmosphere and equipment.

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Authors and Affiliations

  1. Department of Applied Biology, University of Helsinki, P.O. Box 27, Latokartanonkaari 7, FIN-00014, Helsinki, Finland

    Malin Bomberg & Sari Timonen

  2. Department of Applied Chemistry and Microbiology, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland

    Sari Timonen

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  1. Malin Bomberg
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  2. Sari Timonen
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Correspondence to Sari Timonen.

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Bomberg, M., Timonen, S. Distribution of Cren- and Euryarchaeota in Scots Pine Mycorrhizospheres and Boreal Forest Humus. Microb Ecol 54, 406–416 (2007). https://doi.org/10.1007/s00248-007-9232-3

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  • DOI: https://doi.org/10.1007/s00248-007-9232-3

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