Soil Bacteria are Differentially Affected by the Resin of the Medicinal Plant Pseudognaphalium vira vira and Its Main Component Kaurenoic Acid
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The diterpenoid kaurenoic acid is the main component of the resin from the medicinal plant Pseudognaphalium vira vira. As some diterpenoids have antimicrobial properties, the effect of this resin and the kaurenoic acid on soil bacteria was studied. The resin of P. vira vira and purified kaurenoic acid were two to four times more effective as antibacterial agents with Gram-positive than with Gram-negative soil isolates. The chemical stability of kaurenoic acid and the antibacterial activity of both the resin and the diterpenoid were studied in microcosms containing plant-associated soil. After 15 days of incubation, the diterpenoid was stable, as determined by 1H nuclear magnetic resonance and thin-layer chromatography, and soil extracts still exhibited antibacterial activity. However, after 30 days of incubation, loss of antibacterial activity of soil extracts correlated with removal or chemical modification of kaurenoic acid. The effect of the resin or this diterpenoid on the soil bacteria community was analyzed by the terminal restriction fragment length polymorphisms technique. After 15 days of incubation, the resin and the pure compound caused significant changes in the soil bacterial community. The relative abundance of specific bacterial groups was differentially affected by the resin components, being the effects with the resin stronger than with the kaurenoic acid. After 30 days of incubation, these changes mostly reverted. These results indicate that a plant resin containing diterpenoid compounds plays a significant role controlling specific groups of microorganisms in the soil associated with the plant.
KeywordsKaurenoic acid soil bacteria diterpenoids Pseudognaphalium vira vira antibacterial activity
This work was supported by Dirección de Investigación Científica y Tecnológica (DICYT) from the Universidad de Santiago de Chile and by FONDAP-FONDECYT (grant 1501-0001, program 7). R.D.I. is a MECESUP Ph.D. fellow.
- 4.Cole, JR, Chai, B, Marsh, TL, Farris, RJ, Wang, Q, Kulam, SA, Chandra, S, McGarrell, DM, Schmidt, TM, Garrity, GM, Tiedje, JM (2003) The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31: 442–443PubMedCrossRefGoogle Scholar
- 5.Ghisalberti, E (1997) The biological activity of naturally occurring kaurane diterpenes. Fitoterapia 68: 303–324Google Scholar
- 8.Hoffmann, A, Farga, C, Lastra, J, Veghazi, E (1992) Plantas medicinales de uso común en Chile. Fundación Claudio Gay, ChileGoogle Scholar
- 11.Knowles, R (1997) Beneficial microbial soil and plant interactions. In: Martins, MT, Sato, MIZ, Tiedje, JM, Hagler, LCN, Döbereiner, J, Sanchez, PS (Eds.) Progress in Microbial Ecology, Brazilian Society for Microbiology, São Paulo, pp 355–359Google Scholar
- 13.Margalef, R (1958) Information theory in ecology. Gen Syst 3: 36–71Google Scholar
- 14.Massieux, B, Boivin, ME, van den Ende, FP, Langenskiöld, J, Marvan, P, Barranguet, C, Admiraal, W, Laanbroek, HJ, Zwart, G (2004) Analysis of structural and physiological profiles to assess the effects of Cu on biofilm microbial communities. Appl Environ Microbiol 70: 4512–4521PubMedCrossRefGoogle Scholar