Microbial Ecology

, Volume 52, Issue 1, pp 19–25 | Cite as

Degradation of Tributyltin in Microcosm Using Mekong River Sediment

  • Fujiyo Suehiro
  • Takeshi Kobayashi
  • Lisa Nonaka
  • Bui Cach Tuyen
  • Satoru SuzukiEmail author


The degradation of tributyltin (TBT) and changes of bacterial number and community structures were investigated in microcosms using the sediment collected from the Mekong River, Vietnam. Concentrations of TBT in sediments were less than 0.62 ng/g (dry wt), lower than those reported from other areas. TBT-resistant bacteria were found in the three sampling sites, and the occurrence rates were 11–16% out of the total viable count. In this microcosm experiment, initial concentration of TBT [1.0–1.4 μg/g (dry wt)] decreased to 0.6 μg/g (dry wt) during 150 days, whereas that in the control microcosm with autoclaved sediment did not change, indicating that Mekong River sediment contains high TBT-degrading activity by microorganisms. The occurrence of TBT-resistant bacteria and the bacterial community structures monitored by denaturing gradient gel electrophoresis were almost the same between test and control groups, indicating that the addition of TBT had little influence on microbial community structure. Mekong River sediment seems to have a stable microbial community against TBT pollution.


Microbial Community Structure Bacterial Community Structure Tributyltin Mekong Delta Multidrug Efflux Pump 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was partly supported by Research Revolution (RR2002) and 21st Century COE Programs, from the Ministry of Education, Culture, Science, Sports and Technology (MEXT), Japan, and Grants-in-Aid for Scientific Research (14208063, 15201006) from the Japanese Society of Promotion of Science (JSPS).


  1. 1.
    Barug, D (1981) Microbial degradation of bis (tributyltin) oxide. Chemosphere 10: 1145–1154CrossRefGoogle Scholar
  2. 2.
    Cooney, JJ (1995) Organotin compounds and aquatic bacteria: a review. Helgol Meeresunters 49: 663–677CrossRefGoogle Scholar
  3. 3.
    Clark, EA, Sterritt, RM, Lester, JN (1988) The fate of tributyltin in the aquatic environment. Environ Sci Technol 22: 600–604CrossRefGoogle Scholar
  4. 4.
    Dowson, PH, Bubb, JM, Lester, JN (1996) Persistence and degradation pathways of tributyltin in freshwater and estuarine sediments. Estuar Coast Shelf Sci 42: 551–562CrossRefGoogle Scholar
  5. 5.
    Environment Agency of Japan (1998) Endocrine Disrupting Chemicals Interim Investigation Manual (water, sediment, and aquatic organisms).Google Scholar
  6. 6.
    Fent, K (1996) Organotin compounds in municipal wastewater and sewage sludge: contamination, fate in treatment process and ecotoxicological consequences. Sci Total Environ 185: 151–159CrossRefGoogle Scholar
  7. 7.
    Fu, J, Mai, B, Sheng, G, Zhang, G, Wang, X, Peng, P, Xiao, X, Ran, R, Cheng, F, Peng, X, Wang, Z, Tang, UW (2003) Persistent organic pollutants in environment of the Pearl River Delta, China: an overview. Chemosphere 52: 1411–1422PubMedCrossRefGoogle Scholar
  8. 8.
    Fukagawa, T, Suzuki, S, Fukunaga, K, Suzuki, T, Takama, K (1992) Isolation and characterization of tributyltin chloride-resistant marine Vibrio. FEMS Microbiol Lett 93: 83–86CrossRefGoogle Scholar
  9. 9.
    Fukagawa, T, Konno, S, Takama, K, Suzuki, S (1994) Occurrence of tributyltin (TBT) and methyl mercury tolerant bacteria in natural seawater to which TBT was added. J Mar Biotechnol 1: 211–214Google Scholar
  10. 10.
    Gadd, GM (2000) Microbial interactions with tributyltin compounds: detoxification, accumulation, and environmental fate. Sci Total Environ 258: 119–127PubMedCrossRefGoogle Scholar
  11. 11.
    Gillan, DC (2004) The effect of an acute copper exposure on the diversity of a microbial community in North Sea sediments as revealed by DGGE analysis—the importance of the protocol. Mar Pollut Bull 49: 504–513PubMedCrossRefGoogle Scholar
  12. 12.
    Hallas, LE, Cooney, JJ (1981) Tin and tin-resistant microorganisms in Chesapeake Bay. Appl Environ Microbiol 41: 446–471Google Scholar
  13. 13.
    Harino, H, Fukushima, M, Kurokawa, Y, Kawai, S (1997) Degradation of the tributyltin compounds by the microorganisms in water and sediment collected from the harbour area of Osaka City, Japan. Environ Pollut 98: 163–167CrossRefGoogle Scholar
  14. 14.
    Hoch, M (2001) Organotin compounds in the environment—an overview. Appl Geochem 16: 719–743CrossRefGoogle Scholar
  15. 15.
    IMO (2001) Anti-Fouling Convention. International Maritime Organization. Available from:
  16. 16.
    Inoue, H, Takimura, O, Fuse, H, Murakami, K, Kamimura, K, Yamaoka, Y (2003) Degradation of triphenyltin by a fluorescent pseudomonad. Appl Environ Microbiol 66: 3492–3498CrossRefGoogle Scholar
  17. 17.
    Inoue, H, Takimura, O, Kawaguchi, K, Nitoda, T, Fuse, H, Murakami, K, Yamaoka, Y (2003) Tin-carbon cleavage of organotin compounds by pyoverdine from Pseudomonas chlororaphis. Appl Environ Microbiol 69: 878–883PubMedCrossRefGoogle Scholar
  18. 18.
    Inoue, H, Takimura, O, Yamaoka, Y (2003) Degradation of organotin compounds by Burkholderia cepacia CNR22. J Jpn Soc Water Environ 29: 775–778CrossRefGoogle Scholar
  19. 19.
    Jude, F, Arpin, C, Brachet-Castang, C, Capdepuy, M, Caumette, P, Quentin, C (2004) TbtABM, a multidrug efflux pump associated with tributyltin resistance in Pseudomonas stutzeri. FEMS Microbiol Lett 232: 7–14PubMedCrossRefGoogle Scholar
  20. 20.
    Jude, F, Lascourreges, JF, Capdepuy, M, Quentin, C, Caumette, P (1996) Evaluation of tributyltin resistance in marine sediment bacteria. Can J Microbiol 42: 525–532CrossRefGoogle Scholar
  21. 21.
    Langston, WJ, Pope, ND (1995) Determinants of TBT adsorption and desorption in estuarine sediments. Mar Pollut Bull 31: 32–43CrossRefGoogle Scholar
  22. 22.
    Laughlin, Jr, RB, Guard, HE, Coleman, III, WM (1986) Tributyltin in seawater: speciation and octanol–water partition coefficient. Environ Sci Technol 20: 201–204CrossRefGoogle Scholar
  23. 23.
    Ma, H, Dai, S, Huang, G (2000) Distribution of tributyltin chloride in laboratory simulated estuarine microcosms. Water Res 34: 2829–2841CrossRefGoogle Scholar
  24. 24.
    Midorikawa, S, Arai, T, Harino, H, Ohji, M, Cu, ND, Miyazaki, N (2004) Concentrations of organotin compounds in sediment and clams collected from coastal areas in Vietnam. Environ Pollut 131: 401–408PubMedCrossRefGoogle Scholar
  25. 25.
    Muyzer, G, De Waal, EC, Uitterlinden, AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes cording for 16S rRNA. Appl Environ Microbiol 59: 695–700PubMedGoogle Scholar
  26. 26.
    Saeki, K, Nakatani, N, Le, THL, Tanabe, S (1998) Determination of the total tin in biological materials by ICP-MS. Bunseki Kagaku 47: 135–139Google Scholar
  27. 27.
    Seligman, PF, Valkirs, AO, Lee, RF (1986) Degradation of tributyltin in San Diego Bay, California, waters. Environ Sci Technol 20: 1229–1235CrossRefGoogle Scholar
  28. 28.
    Sudaryanto, A, Takahashi, S, Iwata, H, Tanabe, S, Muchtar, M, Razak, H (2005) Organotin residues and the role of anthropogenic tin sources in the coastal marine environment of Indonesia. Mar Pollut Bull 50: 227–236Google Scholar
  29. 29.
    Suzuki, S, Fukagawa, T (1995) Tributyltin-resistant marine bacteria: a summary of recent work. J Ind Microbiol 14: 154–158PubMedCrossRefGoogle Scholar
  30. 30.
    Suzuki, S, Fukagawa, T, Takama, K (1992) Occurrence of tributyltin-tolerant bacteria in tributyltin- or cadmium-containing seawater. Appl Environ Microbiol 58: 3410–3412PubMedGoogle Scholar
  31. 31.
    Takahashi, S, Mukai, H, Tanabe, S, Sakayama, K, Miyazaki, T, Masuno, H (1999) Butyltin residues in livers of humans and wild terrestrial mammals and in plastic products. Environ Pollut 106: 213–214PubMedCrossRefGoogle Scholar
  32. 32.
    White, JS, Tobin, JM, Cooney, JJ (1999) Organotin compounds and their interaction with microorganisms. Can J Microbiol 45: 541–554PubMedCrossRefGoogle Scholar
  33. 33.
    Wuertz, S, Miller, CE, Pfister, RM, Cooney, JJ (1991) Tributyltin-resistant bacteria from estuarine and freshwater sediments. Appl Environ Microbiol 57: 2783–2789PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Fujiyo Suehiro
    • 1
  • Takeshi Kobayashi
    • 1
  • Lisa Nonaka
    • 1
  • Bui Cach Tuyen
    • 2
  • Satoru Suzuki
    • 1
    Email author
  1. 1.Center for Marine Environmental Studies (CMES)Ehime UniversityEhimeJapan
  2. 2.Nong Lam UniversityHo Chi Minh City,Vietnam

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