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Microbial Ecology

, Volume 52, Issue 1, pp 26–33 | Cite as

Temperature Affects Stoichiometry and Biochemical Composition of Escherichia coli

  • James B. CotnerEmail author
  • Wataru Makino
  • Bopaiah A. Biddanda
Article

Abstract

Temperature is a master variable controlling biochemical processes in organisms, and its effects are manifested on many organizational levels in organisms and ecosystems. We examined the effects of temperature on the biochemical composition and stoichiometry of a model heterotrophic bacterium, Escherichia coli K-12, held at constant growth rate in chemostats. Increasing temperature led to increased cellular organic carbon (C) and organic nitrogen (N) with decreased phosphorus (P) content, leading to increased C/P and N/P biomass ratios. P content was related to cellular RNA, which is P-rich (9–10% by weight) and nonnucleic acid P (presumably composed of mostly phospholipids, intracellular phosphate, and polyphosphate). These results indicate that E. coli allocates an increased proportion of its P cell quota toward assembly (ribosomes) at low temperatures and an increasing proportion toward resource acquisition machinery (membranes) at higher temperatures. If these results are relevant to the behavior of prokaryotic heterotrophs in natural settings (the gut, soils, lakes, oceans, etc.), it suggests greater nutrient regeneration and less microbial nutrient retention as temperatures increase.

Keywords

Temperature Stoichiometry Phosphorus Ribosomes DNA RNA E. Coli 

Notes

Acknowledgments

This work was supported by grants from the US-NSF (DEB-9977047 and OCE-9416614), and NOAA (46290000). Ed Hall and Ted Stets provided comments on a previous version of this manuscript.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • James B. Cotner
    • 1
    Email author
  • Wataru Makino
    • 1
    • 2
  • Bopaiah A. Biddanda
    • 1
    • 3
  1. 1.Department of Ecology, Evolution and BehaviorUniversity of MinnesotaSt. PaulUSA
  2. 2.Graduate School of Life ScienceTohoko UniversitySendaiJapan
  3. 3.Annis Water Resources InstituteGrand Valley State UniversityMuskegonUSA

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