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Dynamics of Sulfate-Reducing Microorganisms (dsrAB genes) in Two Contrasting Mudflats of the Seine Estuary (France)

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Abstract

By combining molecular biology and biochemical approaches, the dynamics of sulfate-reducing microorganisms (SRM) was investigated in the sediments of the Seine estuary (France). Both intertidal mixing-zone and freshwater mudflats were sampled during a 1-year period; the quantification of SRM was realized by using competitive polymerase chain reaction (PCR) based on dsrAB gene amplification, previously described by Leloup et al. (2004), and sulfate reduction rate (SRR) was determined via the SO4 2 radiotracer method. Throughout the year, abundance of dsrAB genes and SRR were predominantly high in the top 15 cm of the sediment. A seasonal dynamic was observed; a predominance of activity was noted during the early summer, and seems to be mainly controlled by physical–chemical parameters (temperature and dissolved organic carbon concentration) and topographic evolution of the mudflat (erosion/deposit erosion).

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Acknowledgments

This work was supported by the Seine-Aval scientific research program, grants from the French government, region Haute Normandie, Agence de l'eau, and industrial partners in the Haute Normandie region (France). We thank Ms. D. Moscato for her help with the text (English) of this article. The first author held a research grant from the region of Haute Normandie.

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Authors and Affiliations

  1. Max-Planck Institute for Marine Microbiology, Bremen, Germany

    J. Leloup

  2. Laboratoire de Microbiolologie Du Froid, Groupe Biodiversité et Environment, UPRESS-2123, Université de Rouen, 76821, Mont Saint Aignan, France

    J. Leloup, F. Petit & L. Quillet

  3. Laboratoire d'études radioécologiques de la façade Atlantique, IRSN, Cherbourg-Octeville, France

    D. Boust

  4. Laboratoire de morphodynamique continentale et côtière, UMR CNRS 6143, Université de Rouen, 76821, Mont Saint Aignan, France

    J. Deloffre & G. Bally

  5. Laboratoire de Chimie Analytique Marine, UMR CNRS 8013, Université des Sciences et Technologies de Lille, 59655, Villenueve d'Ascq, France

    O. Clarisse

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  1. J. Leloup
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  2. F. Petit
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  3. D. Boust
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  4. J. Deloffre
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  5. G. Bally
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Correspondence to J. Leloup.

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Leloup, J., Petit, F., Boust, D. et al. Dynamics of Sulfate-Reducing Microorganisms (dsrAB genes) in Two Contrasting Mudflats of the Seine Estuary (France). Microb Ecol 50, 307–314 (2005). https://doi.org/10.1007/s00248-004-0034-6

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