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Response of Biofilm Bacteria to Dissolved Organic Matter from Decomposing Maple Leaves

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Abstract

Stream bacteria play an important role in the utilization of dissolved organic matter (DOM) leached from leaves, and in transfer of this DOM to other trophic levels. Leaf leachate is a mixture of labile, recalcitrant, and inhibitory compounds, and bacterial communities vary in their ability to utilize leachate. The purpose of this study was to determine the effects of DOM from sugar maple leaves on bacterial populations in biofilms on decomposing leaf surfaces. Populations of Acinetobacter calcoaceticus, Burkholderia cepacia, and Pseudomonas putida were enumerated on decomposing maple leaves in a northeast Ohio stream using fluorescence in situ hybridization. Additionally, artificial substrata consisting of PVC-end caps filled with agar supplemented with leaf leachate and covered with cellulose filters were used to determine bacterial response to leachate from leaves at different stages of decomposition. Population sizes of bacterial species exhibited different responses. Leachate did not affect A. calcoaceticus. B. cepacia was tolerant of phenolic compounds released from leaves and the population size increased when DOM concentrations were greatest. In contrast, P. putida was inhibited by phenolic components of leachate when total DOM concentrations were greatest. Differences in response of the bacterial species to components of leaf leachate indicate the complexity of microbial population dynamics and interactions with DOM. Differences among species in response to DOM have the potential to influence transport and retention of organic matter in stream ecosystems.

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References

  1. IT Baldwin JC Schultz (1984) ArticleTitleTannins lost from sugar maple (Acer saccharum) and yellow birch (Betula allegheniensis Britt.) leaf litter. Soil Biol Biochem 16 421–422 Occurrence Handle10.1016/0038-0717(84)90044-0 Occurrence Handle1:CAS:528:DyaL2cXlvFSnsLs%3D

    Article  CAS  Google Scholar 

  2. IT Baldwin JC Schultz D Ward (1987) ArticleTitlePatterns and sources of leaf tannin variation in yellow birch (Betula allegheniensis) and sugar maple (Acer saccharum). J Chem Ecol 13 1069–1078 Occurrence Handle1:CAS:528:DyaL2sXkt1SkurY%3D

    CAS  Google Scholar 

  3. EC Bate-Smith (1962) ArticleTitleThe phenolic constituents of plants and their taxonomic significance. I. Dicotyledons. J Linn Soc (Bot) 58 95–173 Occurrence Handle1:CAS:528:DyaF3sXkslKluro%3D

    CAS  Google Scholar 

  4. TJ Battin A Butturini F Sabater (1999) ArticleTitleImmobilization and metabolism of dissolved organic carbon by natural sediment biofilms in a Mediterranean and temperate stream. Aquat Microb Ecol 19 297–305

    Google Scholar 

  5. Benfield, EF (1997) Comparisons of litterfall input to streams. In: Webster, JR, Meyer, JL (Eds.) Stream Organic Matter Budgets. J N Am Benthol Soc 16:3–161

    Google Scholar 

  6. R Benner ER Peele RE Hodson (1986) ArticleTitleMicrobial utilization of dissolved organic matter from leaves of the Red Mangrove, Rhizophora mangle, in the Fresh Creek Estuary, Bahamas. Estuar Coast Shelf Sci 23 607–619 Occurrence Handle1:CAS:528:DyaL2sXjvVSgsQ%3D%3D

    CAS  Google Scholar 

  7. RE Benoit RL Starkey (1968) ArticleTitleEnzyme inactivation as a factor in the inhibition of decomposition of organic matter by tannins. Soil Sci 105 203–208 Occurrence Handle1:CAS:528:DyaF1cXhtFWkt7s%3D

    CAS  Google Scholar 

  8. EB Braun-Howland PA Vescio SA Nierzwicki-Bauer (1993) ArticleTitleUse of a simplified cell blot technique and 16S rRNA-directed probes for identification of common environmental isolates. Appl Environ Microbiol 59 3219–3224 Occurrence Handle1:CAS:528:DyaK2cXks1OqtQ%3D%3D Occurrence Handle7504429

    CAS  PubMed  Google Scholar 

  9. KW Cummins (1974) ArticleTitleStructure and function of stream ecosystems. BioScience 24 631–641

    Google Scholar 

  10. K Cummins WRC Petersen FO Howard JC Wuycheck VI Holt (1973) ArticleTitleThe utilization of leaf litter by stream detritivores. Ecology 54 336–345

    Google Scholar 

  11. CN Dahm (1981) ArticleTitlePathways and mechanisms for removal of dissolved organic carbon from leaf leachate in streams. Can J Fish Aquat Sci 38 68–76 Occurrence Handle1:CAS:528:DyaL3MXkt12qu78%3D

    CAS  Google Scholar 

  12. RT Edwards (1987) ArticleTitleSestonic bacteria as a food source for filtering invertebrates in two southeastern blackwater rivers. Limnol Oceanogr 32 221–234 Occurrence Handle1:CAS:528:DyaL2sXhvVahs78%3D

    CAS  Google Scholar 

  13. RT Edwards JL Meyer (1987) ArticleTitleBacteria as a food source for black fly larvae in a blackwater river. J N Am Benthol Soc 6 241–250

    Google Scholar 

  14. JW Elwood PJ Mulholland JD Newbold (1988) ArticleTitleMicrobial activity and phosphorus uptake on decomposing leaf detritus in a heterotrophic stream. Verh Internat Verein Limnol 23 1198–1208

    Google Scholar 

  15. P Ferianc D Toth (1995) ArticleTitleMicrobial utilization of black poplar (Populus nigra L.) leaf leachate. Biologia, Bratislava 50 535–541

    Google Scholar 

  16. SG Findlay TL Arsuffi (1989) ArticleTitleMicrobial growth and detritus transformations during decomposition of leaf litter in a stream. Freshwat Biol 21 261–269

    Google Scholar 

  17. SG Fisher GE Likens (1973) ArticleTitleEnergy flow in Bear Brook, New Hampshire: an integrative approach to stream ecosystem metabolism. Ecol Monograph 43 421–439

    Google Scholar 

  18. GG Geesey R Mutch JW Costerton (1978) ArticleTitleSessile bacteria: an important component of the microbial population in small mountain streams. Limnol Oceanogr 23 1214–1223 Occurrence Handle1:CAS:528:DyaE1MXht1OhsLk%3D

    CAS  Google Scholar 

  19. AE Greenberg (1992) Standard Methods for the Examination of Water and Wastewater, 18th ed. American Public Health Association

    Google Scholar 

  20. JA Irvine NJ Dix RC Warren (1978) ArticleTitleInhibitory substances in Acer platanoides leaves: seasonal activity and effects on growth of phylloplane fungi. Trans Br Mycol Soc 3 363–371

    Google Scholar 

  21. E Juni (1978) ArticleTitleGenetics and physiology of Acinetobacter. Ann Rev Microbiol 32 349–371 Occurrence Handle10.1146/annurev.mi.32.100178.002025 Occurrence Handle1:CAS:528:DyaE1cXlvFemsLo%3D

    Article  CAS  Google Scholar 

  22. LA Kaplan TL Bott (1983) ArticleTitleMicrobial heterotrophic utilization of dissolved organic matter in a piedmont stream. Freshwat Biol 13 363–377

    Google Scholar 

  23. NK Kaushik HBN Hynes (1971) ArticleTitleThe fate of the dead leaves that fall into streams. Arch Hydrobiol 68 465–525

    Google Scholar 

  24. KT Killingbeck DL Smith RG Marzolf (1982) ArticleTitleChemical changes in tree leaves during decomposition in a tallgrass prarie stream. Ecology 63 585–589 Occurrence Handle1:CAS:528:DyaL38XitVCqurs%3D

    CAS  Google Scholar 

  25. P Koetsier JV McArthur L Leff (1997) ArticleTitleSpatial and temporal response of stream bacteria to sources of dissolved organic carbon in a blackwater stream system. Freshwat Biol 37 79–89 Occurrence Handle10.1046/j.1365-2427.1997.d01-535.x

    Article  Google Scholar 

  26. LG Leff (2000) ArticleTitleLongitudinal changes in microbial assemblages of the Ogeechee River. Freshwat Biol 43 605–615

    Google Scholar 

  27. LG Leff CJ McNamara MJ Lemke (2003) ArticleTitleBacterial populations of the floodplain of a South Carolina (USA) stream: a comparison of two species. Arch Hydrobiol 156 255–270 Occurrence Handle10.1127/0003-9136/2003/0156-0255

    Article  Google Scholar 

  28. MJ Lemke BJ Brown LG Leff (1997) ArticleTitleThe response of three bacterial populations to pollution in a stream. Microb Ecol 34 224–231 Occurrence Handle10.1007/s002489900051 Occurrence Handle1:CAS:528:DyaK2sXmvFegs74%3D Occurrence Handle9337417

    Article  CAS  PubMed  Google Scholar 

  29. MJ Lemke CJ McNamara LG Leff (1997) ArticleTitleComparison of methods for in situ hybridization of bacterioplankton. J Microbiol Meth 29 23–29 Occurrence Handle10.1016/S0167-7012(97)00989-5 Occurrence Handle1:CAS:528:DyaK2sXksVSrtbk%3D

    Article  CAS  Google Scholar 

  30. MJ Lemke LG Leff (1999) ArticleTitleBacterial populations in an anthropogenically disturbed stream: comparison of different seasons. Microb Ecol 38 234–243 Occurrence Handle10.1007/s002489900173 Occurrence Handle10541785

    Article  PubMed  Google Scholar 

  31. TG Lessie T Gaffney (1986) Catabolic potential of Pseudomonas cepacia. JR Sokatch (Eds) The Bacteria: A Treatise on Structure and Function Academic Press New York 439–481

    Google Scholar 

  32. J Liu LG Leff (2002) ArticleTitleTemporal changes in the bacterial community of a Northeast Ohio (USA) river. Hydrobiologia 489 151–159 Occurrence Handle10.1023/A:1023228703738 Occurrence Handle1:STN:280:DC%2BD3svnsFOktg%3D%3D Occurrence Handle14552350

    Article  CAS  PubMed  Google Scholar 

  33. MA Lock RR Wallace JW Costerton RM Ventullo SE Charlton (1984) ArticleTitleRiver epilithon: toward a structural functional model. Oikos 42 10–22

    Google Scholar 

  34. JV McArthur GR Marzolf JE Urban (1985) ArticleTitleResponse of bacteria isolated from a pristine prairie stream to concentration and source of soluble organic carbon. Appl Environ Microbiol 49 238–241

    Google Scholar 

  35. JV McArthur GR Marzolf (1986) ArticleTitleInteractions of the bacterial assemblages of a prairie stream with dissolved organic carbon from riparian vegetation. Hydrobiologia 134 193–199 Occurrence Handle1:CAS:528:DyaL28XkvVCltrw%3D

    CAS  Google Scholar 

  36. CJ McNamara MJ Lemke LG Leff (2002) ArticleTitlePopulation sizes of three bacterial species in sediments of a South Carolina stream. Hydrobiologia 482 151–159 Occurrence Handle10.1023/A:1021268516231

    Article  Google Scholar 

  37. JL Meyer (1994) ArticleTitleThe microbial loop in flowing waters. Microb Ecol 28 195–199 Occurrence Handle1:CAS:528:DyaK2MXit1Wktb8%3D

    CAS  Google Scholar 

  38. Ohio Environmental Protection Agency Technical Report MAS/1995-12-14. Biological and water quality study of the Mahoning River Basin

  39. T Saito (1957) ArticleTitleChemical changes in beech litter under microbial decomposition. Ecol Rev 3 209–216

    Google Scholar 

  40. KH Schleifer R Amann W Ludwig C Rothemund N Springer S Dorn (1992) Nucleic acid probes for the identification and in situ detection of pseudomonads. E Galli S Silver B Witholt (Eds) Pseudomonas: Molecular Biology and Biotechnology American Society for Microbiology Washington, DC 127–134

    Google Scholar 

  41. RY Stanier NJ Palleroni M Doudoroff (1966) ArticleTitleThe aerobic Pseudomonads: a taxonomic study. J Gen Microbiol 43 159–271 Occurrence Handle1:STN:280:CCiC38fhtlU%3D Occurrence Handle5963505

    CAS  PubMed  Google Scholar 

  42. K Suberkropp MJ Klug (1976) ArticleTitleFungi and bacteria associated with leaves during processing in a woodland stream. Ecology 57 707–719

    Google Scholar 

  43. EM Thurman (1985) Organic Geochemistry of Natural Waters DR W. Junk Boston

    Google Scholar 

  44. Treadwell, SA, Campbell, IC, Edwards, RT (1997) Organic matter dynamics in Keppel Creek, southeastern Australia. In: Webster, JR, Meyer, JL (Eds.) Stream Organic Matter Budgets, J N Am Benthol Soc 16:3–161

  45. RL Vannote WG Minshall KW Cummins JR Sedell CE Gushing (1980) ArticleTitleThe river continuum concept. Can J Fish Aquat Sci 37 130–137

    Google Scholar 

  46. PG Waterman S Mole (1994) Analysis of Phenolic Plant Metabolites Blackwell Scientific Publications Oxford

    Google Scholar 

  47. WV Zucker (1983) ArticleTitleTannins: does structure determine function? An ecological perspective. Am Natural 121 335–365 Occurrence Handle10.1086/284065 Occurrence Handle1:CAS:528:DyaL3sXhsVSmsrg%3D

    Article  CAS  Google Scholar 

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Acknowledgments

This research was supported by the Department of Biological Sciences, Kent State University, and Sigma Xi Grants-in-Aid of Research.

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  1. C.J. McNamara

    Present address: Laboratory of Microbial Ecology, Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA

Authors and Affiliations

  1. Department of Biological Sciences, Kent State University, Kent, OH, 44242, USA

    C.J. McNamara & L.G. Leff

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Correspondence to C.J. McNamara.

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McNamara, C., Leff, L. Response of Biofilm Bacteria to Dissolved Organic Matter from Decomposing Maple Leaves. Microb Ecol 48, 324–330 (2004). https://doi.org/10.1007/s00248-003-1058-z

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